Vector Labs Ab03109-23.0 Anti-FimA [256-265-9], Rabbit IgG, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 256-265-9
Synonyms: Major fimbrium subunit FimA type-1; Fimbrillin; Fimbrilin; Major fimbrial subunit protein type I; fimbrial protein; fimbriae protein
Amount: 200 μg
Application Codes Clone: ELISA; WB; inhibition
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: The antibody reacts with conformational epitopes displayed by partially dissociated oligomers of FimA, but not with the 43 kDa FimA monomer. FimA is a structural protein of P. gingivalis fimbriae and is known to be involved in bacterial adherence to the host cell surface.
Application Notes: The antibody reacted with conformational epitopes expressed by partially dissociated oligomers, but not with monomer as elucidated by Western blot analysis. Ascites fluid containing the antibody showed the inhibitory activity against P. gingivalis to saliva-coated hydroxyapatite beads, an in vitro model for the pellicle-coated tooth surface. For example, 400-fold diluted monoclonal antibody inhibited the binding of the bacteria onto bead surfaces of 41.5%,(Koh et al., 2009; PMID: 20157608). The specificity of this antibody (IgG1) was confirmed by ELISA analysis. The original antibody detected FimA by western blot. The antibody significantly inhibited the binding of P. gingivalis to the surface of SHA beads by 51.81 ± 2.17%, when 50 ng/mL antibody was used (P < 0.01). The antibody could be used as a basis for passive immunization against P. gingivalis-mediated periodontitis (Koh et al., 2011; PMID: 21223367).

Product Class:	Purified
Clone ID:	256-265-9
Synonyms:	Major fimbrium subunit FimA type-1; Fimbrillin; Fimbrilin; Major fimbrial subunit protein type I; fimbrial protein; fimbriae protein
Amount:	200 μg
Application Codes Clone:	ELISA; WB; inhibition
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	The antibody reacts with conformational epitopes displayed by partially dissociated oligomers of FimA, but not with the 43 kDa FimA monomer. FimA is a structural protein of P. gingivalis fimbriae and is known to be involved in bacterial adherence to the host cell surface.
Application Notes:	The antibody reacted with conformational epitopes expressed by partially dissociated oligomers, but not with monomer as elucidated by Western blot analysis. Ascites fluid containing the antibody showed the inhibitory activity against P. gingivalis to saliva-coated hydroxyapatite beads, an in vitro model for the pellicle-coated tooth surface. For example, 400-fold diluted monoclonal antibody inhibited the binding of the bacteria onto bead surfaces of 41.5%,(Koh et al., 2009; PMID: 20157608). The specificity of this antibody (IgG1) was confirmed by ELISA analysis. The original antibody detected FimA by western blot. The antibody significantly inhibited the binding of P. gingivalis to the surface of SHA beads by 51.81 ± 2.17%, when 50 ng/mL antibody was used (P < 0.01). The antibody could be used as a basis for passive immunization against P. gingivalis-mediated periodontitis (Koh et al., 2011; PMID: 21223367).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

Reviews