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Mfg Part Number

Ab03113-10.0

Vector Labs Ab03113-10.0 Anti-Glycoprotein GPIIb/IIIa complex [314.3], Human IgG1, kappa (200 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by immunizing BALB/c mice with a mixture of quinine-linked GPIIb/IIIa, unlinked GPIIb/IIIa, and soluble quinine.

$517.40

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Description

Product Class: Purified
Clone ID: 314.3
Synonyms: Integrin beta-3; Platelet membrane glycoprotein IIIa; GPIIIa; CD61; integrin αIIbβ3
Amount: 200 μg
Application Codes Clone: FC; Crystallization; in vitro
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: The antibody recognizes the N terminus of the GPIIb beta propeller domain only when soluble quinine is present.
Application Notes: The antibody reacted with normal human platelets in the presence of quinine, as shown by flow cytometry. The reactions of the antibody with CHO cells expressing all-human GPIIb/IIIa (H/H), all-rat GPIIb/IIIa (R/R), or human/rat mixed GPIIb/IIIa integrins (H/R, R/H) were studied by flow cytometry. Quinine dependent antibody binding occurred with constructs H/H and H/R, but not with R/R or R/H (Bougie et al., 2009; PMID: 18948570). The crystal structures of the Fab fragment and its complex with quinine were determined. Quinine induced structural modifications of the Fab fragment; the authors propose that quinine remodels the Fab paratope and enables binding to the platelet GPIIb/IIIa. The monovalent KD values for quinine binding to the IgG fragment measured was 24 nM (Zhu et al., 2015; PMID: 26282540).

Product Class:	Purified
Clone ID:	314.3
Synonyms:	Integrin beta-3; Platelet membrane glycoprotein IIIa; GPIIIa; CD61; integrin αIIbβ3
Amount:	200 μg
Application Codes Clone:	FC; Crystallization; in vitro
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	The antibody recognizes the N terminus of the GPIIb beta propeller domain only when soluble quinine is present.
Application Notes:	The antibody reacted with normal human platelets in the presence of quinine, as shown by flow cytometry. The reactions of the antibody with CHO cells expressing all-human GPIIb/IIIa (H/H), all-rat GPIIb/IIIa (R/R), or human/rat mixed GPIIb/IIIa integrins (H/R, R/H) were studied by flow cytometry. Quinine dependent antibody binding occurred with constructs H/H and H/R, but not with R/R or R/H (Bougie et al., 2009; PMID: 18948570). The crystal structures of the Fab fragment and its complex with quinine were determined. Quinine induced structural modifications of the Fab fragment; the authors propose that quinine remodels the Fab paratope and enables binding to the platelet GPIIb/IIIa. The monovalent KD values for quinine binding to the IgG fragment measured was 24 nM (Zhu et al., 2015; PMID: 26282540).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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