Vector Labs Ab03121-10.3 Anti-XCR1 [5G7], Human IgG1, Fc Silent™, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 5G7
Heavy Chain Modification: Fc Silent™
Synonyms: CCXCR1; GPR5; chemokine XC receptor 1; G-protein coupled receptor 5; lymphotactin receptor
Amount: 200 μg
Application Codes Clone: ELISA; FC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2b
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody binds human XCR1, more particularly it strongly binds amino acids 'PESTTFFYYDLQ' of the human XCR1. XRC1 is a receptor for chemokines SCYC1 and SCYC2 and expressed on 80% of CD8+ the dendritic cells. It also acts as a receptor for XCL1/Lymphotactin. It transduces a signal by increasing the intracellular calcium ions level.
Application Notes: This antibody is capable of binding human XCR1-EGFP-expressing B300.19 cells in a flow cytometry analysis. The determination of the binding epitope of this antibody was done using ELISA (US9371389). This antibody exhibits a significant effect in treating rheumatoid arthritis in the experiment of delayed-type hypersensitivity reaction induced by Mycobacterium butyricum delayed-type hypersensitivity reaction. The neutralizing activity of this antibody against human lymphotactin-induced migration of human XCR1-expressing cells was evaluated by in vitro chemotaxis assay. It was reported that this antibody completely inhibited cell migration at a concentration of about 3 μg/mL (US9371389). The effect of this antibody on ear swelling of DNFB (Dinitrofluorobenzene)-sensitized mice was done and it was reported that this antibody caused significant suppression of ear swelling 24 after induction. This antibody was also reported to show showed a very low reactivity to human CX3CR1-EGFP-expressing B300.19 cells (US9371389). The original antibody with saporin-conjugated secondary antibody showed growth suppression of human XCR1-EGFP-expressing B300.19 cells. The humanized version of this antibody is also available on request (US9371389).

Product Class:	Purified
Clone ID:	5G7
Heavy Chain Modification:	Fc Silent™
Synonyms:	CCXCR1; GPR5; chemokine XC receptor 1; G-protein coupled receptor 5; lymphotactin receptor
Amount:	200 μg
Application Codes Clone:	ELISA; FC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2b
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody binds human XCR1, more particularly it strongly binds amino acids 'PESTTFFYYDLQ' of the human XCR1. XRC1 is a receptor for chemokines SCYC1 and SCYC2 and expressed on 80% of CD8+ the dendritic cells. It also acts as a receptor for XCL1/Lymphotactin. It transduces a signal by increasing the intracellular calcium ions level.
Application Notes:	This antibody is capable of binding human XCR1-EGFP-expressing B300.19 cells in a flow cytometry analysis. The determination of the binding epitope of this antibody was done using ELISA (US9371389). This antibody exhibits a significant effect in treating rheumatoid arthritis in the experiment of delayed-type hypersensitivity reaction induced by Mycobacterium butyricum delayed-type hypersensitivity reaction. The neutralizing activity of this antibody against human lymphotactin-induced migration of human XCR1-expressing cells was evaluated by in vitro chemotaxis assay. It was reported that this antibody completely inhibited cell migration at a concentration of about 3 μg/mL (US9371389). The effect of this antibody on ear swelling of DNFB (Dinitrofluorobenzene)-sensitized mice was done and it was reported that this antibody caused significant suppression of ear swelling 24 after induction. This antibody was also reported to show showed a very low reactivity to human CX3CR1-EGFP-expressing B300.19 cells (US9371389). The original antibody with saporin-conjugated secondary antibody showed growth suppression of human XCR1-EGFP-expressing B300.19 cells. The humanized version of this antibody is also available on request (US9371389).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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