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Mfg Part Number

Ab03122-10.0

Vector Labs Ab03122-10.0 Anti-PAR2 [Ab4999 (Par-B)], Human IgG1, lambda (200 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated using phage display techniques to pan for antibodies (Fab fragments) capable of immunospecifically binding to residues spanning the protease cleavage site at the N-terminal of PAR-2. The affinity maturation of the selected parental clone Ab3777 resulted in generation of the antibody.

$517.40

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Description

Product Class: Purified
Clone ID: Ab4999 (Par-B)
Synonyms: F2RL1; PAR-2; GPR11; Proteinase-activated receptor 2; Coagulation factor II receptor-like 1; G-protein coupled receptor 11; Thrombin receptor-like 1; Ab3777
Amount: 200 μg
Application Codes Clone: ELISA
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG4
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This reformatted human antibody was made using the variable domain sequences of the original Human IgG4 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody has binding specificity for N-terminal PAR-2 peptide ‘SKGRSLIGKVDGTSHVTGKGV’ and can bind human, mouse, rat and cynomolgus monkey PAR-2 peptide. PAR2 acts as a receptor for trypsin and trypsin-like enzymes coupled to G proteins.
Application Notes: This antibody was generated by the affinity maturation of a parental clone Ab3777. This antibody exhibited concentration-dependent binding to human PAR-2 transfected HEK293 cells with EC50 values of 0.2 nM. ELISA experiments demonstrated specific binding of this antibody to PAR-2 peptide (coupled to Transferrin as carrier). This antibody did not show any binding affinity for PAR-4 peptide or PAR-1 peptide. This antibody showed potent inhibition of calcium release in the human lung epithelial cell line A549 stimulated with trypsin similar to the parental clone Ab3777. (US20110311553).

Product Class:	Purified
Clone ID:	Ab4999 (Par-B)
Synonyms:	F2RL1; PAR-2; GPR11; Proteinase-activated receptor 2; Coagulation factor II receptor-like 1; G-protein coupled receptor 11; Thrombin receptor-like 1; Ab3777
Amount:	200 μg
Application Codes Clone:	ELISA
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG4
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This reformatted human antibody was made using the variable domain sequences of the original Human IgG4 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody has binding specificity for N-terminal PAR-2 peptide ‘SKGRSLIGKVDGTSHVTGKGV’ and can bind human, mouse, rat and cynomolgus monkey PAR-2 peptide. PAR2 acts as a receptor for trypsin and trypsin-like enzymes coupled to G proteins.
Application Notes:	This antibody was generated by the affinity maturation of a parental clone Ab3777. This antibody exhibited concentration-dependent binding to human PAR-2 transfected HEK293 cells with EC50 values of 0.2 nM. ELISA experiments demonstrated specific binding of this antibody to PAR-2 peptide (coupled to Transferrin as carrier). This antibody did not show any binding affinity for PAR-4 peptide or PAR-1 peptide. This antibody showed potent inhibition of calcium release in the human lung epithelial cell line A549 stimulated with trypsin similar to the parental clone Ab3777. (US20110311553).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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