Mfg Part Number
Ab03126-23.0
Vector Labs Ab03126-23.0 Anti-BCMA [I09], Rabbit IgG, kappa (200 μg)
Quick Overview
The original antibody 15B2 was generated by immunizing six week old female Ablexis transgenic mice with both rHu BCMA-Fc and cynomolgus BCMA-Fc or adherent 293 cells (Ad293) expressing either Hu BCMA or Cyno BCMA. The original mouse antibody 15B2 was mutated to a germline form using primers designed to mutate four non-germline residues in 15B2, which resulted in the generation of this lead optimized antibody.
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| Product Class: | Purified |
|---|---|
| Clone ID: | I09 |
| Synonyms: | CD269; BCM; TNFRSF17; Tumor necrosis factor receptor superfamily member 17; B-cell maturation protein; 15B2; IO9 |
| Amount: | 200 μg |
| Application Codes Clone: | FC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | This antibody binds human and cynomolgus monkey BCMA. It acts as a receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. BCMA expression is highest on terminally differentiated B cells. BCMA is involved in mediating the survival of plasma cells for maintaining long-term humoral immunity. It also activates NF-kappa-B and JNK. The expression of BCMA has been linked to a number of cancers, autoimmune disorders, and infectious diseases. |
| Application Notes: | The binding affinity of this antibody to monomeric soluble human BCMA was determined using ProteOn™ XPR36 instrument. It was reported that the original human IgG1 version of the antibody binds soluble human BCMA with an affinity of kd= 9.82nM. The binding of this antibody to membrane bound human BCMA was evaluated using flow cytometry in multiple myeloma and plasma cell leukemia cell lines that endogenously express BCMA (NCI-H929, MM.1S cell lines and Ad293 expressing human BCMA). This antibody was also used in the generation of a antibody-drug conjugate (ADC) comprising a BCMA monoclonal antibody like I09 conjugated to a cytotoxin to check its anti-tumor effects in the treatment of multiple myeloma (US10988546). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |