Mfg Part Number
Ab03129-10.0
Vector Labs Ab03129-10.0 Anti-CD2 [35.1], Human IgG1, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing mice with human T lymphocytes that had been activated with phorbol myristic acid (PMA).
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| Product Class: | Purified |
|---|---|
| Clone ID: | 35.1 |
| Synonyms: | LFA-2; LFA-3 receptor; SRBC; Rosette receptor; T-cell surface antigen CD2; Erythrocyte receptor; T-cell surface antigen T11/Leu-5 |
| Amount: | 200 μg |
| Application Codes Clone: | IP; FC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | This antibody recognizes an epitope on the extracellular domain of the human T cell surface protein Tp50 (CD2) between amino acid residues 39-50. The binding of this antibody does not inhibit the interaction between CD2 and CD58. CD2 is a glycoprotein belonging to the immunoglobulin superfamily. It acts as a specific marker for T lymphocytes, natural killer cells, thymic B cell subsets, and some antigen presenting cells. CD2 interacts with CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function. |
| Application Notes: | This antibody inhibits T cell proliferative responses and cytotoxicity, but does not inhibit antibody mediated cellular cytotoxicity. This antibody was used in the generation of CD2 targeting ricin A chain-containing immunotoxins (PMID: 2461993). This antibody is capable of immunoprecipitating CD2 antigen form cell lysates (PMID: 8557753). This antibody was also used for the cell cycle analysis of cells along with other antibodies like anti-CD3, anti-CD4, and anti-CD8 using flow cytometry (PMID: 9881968). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |