Mfg Part Number
Ab03145-3.3
Vector Labs Ab03145-3.3 Anti-CD20 [ABM58020-32A8-PU], Mouse IgG2b, Fc Silent™, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing BALB/c mice with CD20-KLH conjugate in Freund's complete adjuvant.
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| Product Class: | Purified |
|---|---|
| Clone ID: | ABM58020-32A8-PU |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | Bp35; Leu-16; MS4A1; Leukocyte surface antigen Leu-16; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Membrane-spanning 4-domains subfamily A member 1; L26 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; WB; IHC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody binds human CD20. CD20 is a 33kDa tetraspanning transmembrane phosphoprotein which is expressed in pre B-cells. This B-lymphocyte-specific membrane protein plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes. It also functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR. |
| Application Notes: | This binding specificity of this antibody for human CD20 was checked using ELISA. This antibody positively binds human CD20-BSA in an ELISA. The affinity constant of the original IgG2b antibody was determined to be 3.07 × 10^10 M^(-1). This antibody can recognize human CD20 in an immunoblot assay. This antibody is similar to commercially available anti-CD20 antibody L26 in immunohistochemical staining of B cell lymphomas. This antibody was used in the immunohistochemical staining of B cell lymphomas, T cell lymphomas, tonsil tissue and appendectonic tissues (CN111363043). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |