Mfg Part Number
Ab03147-10.3
Vector Labs Ab03147-10.3 Anti-Alpha actin 1 [3G8C7A6], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing BALB/c mice with a recombinant human actin protein.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 3G8C7A6 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | Actin; ACTA1; ACTA; Actin alpha skeletal muscle; Alpha-actin-1; Muscle specific actin; MSA; HHF35; CGMCNO.22312; HHF35 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; IHC-P |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | This antibody binds actin. Actin is a type of spherical multi-functional protein that forms a microfilament. Actin is widely existent in eukaryotic organisms, and there is a major component constituting the muscle segment and the cell skeleton in the cytoplasm and cellular nucleus of the intramuscular cell. Muscle specific actin (MSA) is a highly conserved, ubiquitous protein found in muscle and some non-muscle cells. In addition to participating in the normal morphology of maintaining cells, actin also participates in the formation, adhesion, migration, apoptosis, material transport, transmembrane information transfer, and receptor aggregation. |
| Application Notes: | This antibody is highly similar to the commercially available anti-actin antibody HHF35. This antibody can use used in the immunohistochemical staining of smooth fibroids. This antibody can be used in the identification of actin from various tissues including brain, heart, cerebellum, esophageal, adrenal, stomach, ovaries, small intestines, pancreatic, colorectal, parathyroid, liver, pituitary, salivary gland, testicular, kidney, thyroid, prostate, breast, uterus, spleen, bladder, tonsil, skeletal muscle, thymus (young children), skin, bone marrow, peripheral nerve, lung, melodder cells. The binding specificity of this antibody towards actin was determined using ELISA. The original IgG2b antibody binds actin with an affinity constant of 6.26X10^9 M^(-1) (CN113817055). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |