Mfg Part Number
Ab03149-23.0
Vector Labs Ab03149-23.0 Anti-p16 [30859-S17], Rabbit IgG, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing BALB/c mice with recombinant p16 antigen protein.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 30859-S17 |
| Synonyms: | CDKN2A; CDKN2; CDK4I; MTS-1; p16-INK4a; p16-INK4; p16INK4A; cyclin-dependent kinase inhibitor 2A; Cyclin-dependent kinase 4 inhibitor; Multiple tumor suppressor 1; E6H4 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; WB; IHC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | This antibody binds human p16, which is located in the nucleus and cytoplasm. It acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein. |
| Application Notes: | The original IgG1 version of this antibody binds human p16 protein with a affinity constant of 1.92 X 10^9 M^(-1). The binding specificity and affinity of this antibody for p16 was determined by ELISA. Western blotting experiments suggested that this antibody is capable of recognizing p16 and tumor cells expressing p16 molecules like Hela cells. The immunohistochemical staining pattern of this antibody is highly similar to another anti-p16 antibody E6H4 (CN105001327). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |