Vector Labs Ab03153-23.0 Anti-Carcinoma surface antigen [AC6C3], Rabbit IgG, lambda (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: AC6C3
Synonyms: cancerous cells
Amount: 200 μg
Application Codes Clone: IP; IF; FC; WB
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgM
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgM format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody can recognize antigens expressed on the cell surface of human ovarian, cervix, breast, colon, melanoma and other carcinomas. This antibody does not react with normal human tissues. The epitope recognized by this antibody is different from the one recognized by another similar antibody CR4E8.
Application Notes: This antibody immunoprecipitated a 32 kD polypeptide from lysates of the ovarian tumor cell line SKOV3. Cell surface immunofluorescence determinations using the fluorescence activated cell sorter revealed that this antibody stain ovarian or cervix carcinoma tumor cell lines (PMID: 7756578). Immunoperoxidase staining with this antibody of nonpermeabilized cryostat sections of freshly obtained or cryopreserved ovarian carcinoma specimens and human ovarian tumor xenografts demonstrated strong reactivity of these specimens. This antibody mediated complement-dependent cytotoxicity and identified a 32 Kd band in western blotting and immunoprecipitation experiments conducted on surface labelled SKOV3 cells. The association constant for the IgM version of the antibody was determined at 2.3 x 10^(10) M^(-1) (PMID: 2032733). The biodistribution of 111In-labeled IgM conjugate of this antibody was studied after i.v. or i.p. administration in nude mice bearing an s.c. xenograft or peritoneal tumor lumps of a human colorectal carcinoma (SW620). The i.p. administration of intact, specific radiolabeled IgM provides prolonged retention of radioactivity in tumor, low normal tissue uptake, a long peritoneal residence time, and very limited spillover of IgM into the circulation (PMID:7493338).

Product Class:	Purified
Clone ID:	AC6C3
Synonyms:	cancerous cells
Amount:	200 μg
Application Codes Clone:	IP; IF; FC; WB
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgM
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgM format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody can recognize antigens expressed on the cell surface of human ovarian, cervix, breast, colon, melanoma and other carcinomas. This antibody does not react with normal human tissues. The epitope recognized by this antibody is different from the one recognized by another similar antibody CR4E8.
Application Notes:	This antibody immunoprecipitated a 32 kD polypeptide from lysates of the ovarian tumor cell line SKOV3. Cell surface immunofluorescence determinations using the fluorescence activated cell sorter revealed that this antibody stain ovarian or cervix carcinoma tumor cell lines (PMID: 7756578). Immunoperoxidase staining with this antibody of nonpermeabilized cryostat sections of freshly obtained or cryopreserved ovarian carcinoma specimens and human ovarian tumor xenografts demonstrated strong reactivity of these specimens. This antibody mediated complement-dependent cytotoxicity and identified a 32 Kd band in western blotting and immunoprecipitation experiments conducted on surface labelled SKOV3 cells. The association constant for the IgM version of the antibody was determined at 2.3 x 10^(10) M^(-1) (PMID: 2032733). The biodistribution of 111In-labeled IgM conjugate of this antibody was studied after i.v. or i.p. administration in nude mice bearing an s.c. xenograft or peritoneal tumor lumps of a human colorectal carcinoma (SW620). The i.p. administration of intact, specific radiolabeled IgM provides prolonged retention of radioactivity in tumor, low normal tissue uptake, a long peritoneal residence time, and very limited spillover of IgM into the circulation (PMID:7493338).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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