Mfg Part Number
Ab03155-10.0
Vector Labs Ab03155-10.0 Anti-CD3e [33H1], Human IgG1, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing BALB/c mice with recombinant human CD3 antigen (amino acids 23-126).
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 33H1 |
| Synonyms: | T3E; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3-ε; CGMCC NO 21980; SP7 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; WB; IHC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | This antibody binds human CD3e. It is part of TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. It also plays a role in signal transduction during T cell development and in correct T-cell development. |
| Application Notes: | This binding specificity of this antibody for human CD3e was checked using ELISA. The affinity constant of the original IgG2a antibody was determined to be 1.92×10^9 M^(-1). This antibody can recognize human CD3e recombinant protein in an immunoblot assay. This antibody is similar to commercially available anti-CD3 antibody SP7 in immunohistochemical staining of T cell lymphomas. This antibody can also be used for the immunohistochemical staining of normal tissues like brain, heart, cerebellum, esophageal, adrenal, stomach, ovary, small intestine, pancreatic, colorectal, paratony gland, liver, pituitary, salivary gland, testicular, kidney, thyroid, prostate, breast, uterus, spleen , Bladder, tonsil, skeletal muscle, thymus (young children), skin, bone marrow, peripheral nerve, lung and melodder cells (CN111363043). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |