Vector Labs Ab03158-3.3 Anti-Lipid A [S1-15], Mouse IgG2b, Fc Silent™, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: S1-15
Heavy Chain Modification: Fc Silent™
Synonyms: lipA
Amount: 200 μg
Application Codes Clone: ELISA; Crystallization; EIA; PIH
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2b
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: The antibody recognizes the carbohydrate backbone of lipid A. The antibody binds to the 4'-monophosphoryl GlcN disaccharide. The antibody does not cross react with lipopolysaccharides (LPS). The antibody cross reacts with ssDNA. Lipid A is the lipid component of LPS contained in gram-negative bacteria. Lipid A is the endotoxic principle of LPS.
Application Notes: In passive immunohemolysis, the antibody reacted with bisphosphoryl compounds as well as with the 4'-monophosphoryl partial structures (Kuhn et al., 1992; PMID 1375194). The specificity of the antibody was determined by ELISA analysis. The crystal structures of the Fab fragment in complex with lipid A carbohydrate backbone and in the unliganded form were determined by X-ray diffraction, showing the binding mechanism of the antibody is a lock and key type. Further, the structure of the Fab fragment in complex with single-stranded DNA fragment 5(dT) oligonucleotide was determined. The antibody bound two separate oligonucleotides by their terminal 5- and 3-phosphate groups (Haji-Ghassemi et al., 2015; PMID: 26085093). Solid-phase enzyme immunoassay shown the antibody binds better to the glycoconjugate at low antigen concentrations and better to the lipid A when high concentrations of antigen were used (Brade et al., 1993). The antibody was tested against the synthetic lipid A precursor Ia and compound B1047, a precursor Ia, by enzyme immunoassay. The antibody bound to precursor Ia, but not to compound B 1047 or Escherichia coli Re LPS (Brade et al., 1997; PMID: 9284181).

Product Class:	Purified
Clone ID:	S1-15
Heavy Chain Modification:	Fc Silent™
Synonyms:	lipA
Amount:	200 μg
Application Codes Clone:	ELISA; Crystallization; EIA; PIH
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2b
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	The antibody recognizes the carbohydrate backbone of lipid A. The antibody binds to the 4'-monophosphoryl GlcN disaccharide. The antibody does not cross react with lipopolysaccharides (LPS). The antibody cross reacts with ssDNA. Lipid A is the lipid component of LPS contained in gram-negative bacteria. Lipid A is the endotoxic principle of LPS.
Application Notes:	In passive immunohemolysis, the antibody reacted with bisphosphoryl compounds as well as with the 4'-monophosphoryl partial structures (Kuhn et al., 1992; PMID 1375194). The specificity of the antibody was determined by ELISA analysis. The crystal structures of the Fab fragment in complex with lipid A carbohydrate backbone and in the unliganded form were determined by X-ray diffraction, showing the binding mechanism of the antibody is a lock and key type. Further, the structure of the Fab fragment in complex with single-stranded DNA fragment 5(dT) oligonucleotide was determined. The antibody bound two separate oligonucleotides by their terminal 5- and 3-phosphate groups (Haji-Ghassemi et al., 2015; PMID: 26085093). Solid-phase enzyme immunoassay shown the antibody binds better to the glycoconjugate at low antigen concentrations and better to the lipid A when high concentrations of antigen were used (Brade et al., 1993). The antibody was tested against the synthetic lipid A precursor Ia and compound B1047, a precursor Ia, by enzyme immunoassay. The antibody bound to precursor Ia, but not to compound B 1047 or Escherichia coli Re LPS (Brade et al., 1997; PMID: 9284181).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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