Mfg Part Number
Ab03162-10.0
Vector Labs Ab03162-10.0 Anti-Norovirus GII.10 [5B18], Human IgG1, kappa (200 μg)
Quick Overview
The antibody was generated from a mouse immunized with GII.4 norovirus-445 VLPs.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 5B18 |
| Synonyms: | Capsid protein |
| Amount: | 200 μg |
| Application Codes Clone: | Crystallization; ELISA; WB; IF |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | The antibody binds to a face of the GII.10 P1 subdomain close to the S domain and not openly exposed at the VLP surface (residues Val433, Glu496, Asn530, Tyr533, Thr534, and Leu535). The antibody cross-reacts with three GII genotypes GII.4, GII.10, and GII.12 but not with GI genotypes. |
| Application Notes: | The specificity of the IgG format of this antibody was confirmed by ELISA analysis; the antibody detected intact GII.10 VLPs at different pH values with nearly identical efficacy. The structure of the Fab fragment in complex with GII.10 P domain was determined. The Western blotting results showed that the antibody IgG could detect the three GII genotypes (Hansman et al., 2012; PMID: 22278249). The antibody was used for immunofluorescence microscopy to examine VLP binding to fresh biopsies of human ileum and it bound specifically to VLPs (Murakami et al., 2013; PMID: 23799113). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |