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Mfg Part Number

Ab03168-10.0

Vector Labs Ab03168-10.0 Anti-Kit [79D], Human IgG1, lambda (200 μg)

VECTOR LABS

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Quick Overview

Phage pools consisting of a phage-displayed synthetic antibody library (library F) were cycled through five rounds of selections by using KITD4-5.

$517.40

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Description

Product Class: Purified
Clone ID: 79D
Synonyms: Mast/stem cell growth factor receptor Kit (EC:2.7.10.1); SCFR; Piebald trait protein; PBT; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; CD117
Amount: 200 μg
Application Codes Clone: Crystallography; SPR; inhibition; IP; WB
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: Fab
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: The antibody is specific for KIT, and it binds to the membrane-proximal Ig-like D4 of KIT. The receptor tyrosine kinase KIT is a transmembrane protein that plays crucial roles in mediating diverse cellular processes, including cell differentiation, proliferation, and cell survival, among other activities.
Application Notes: The crystal structure of the Fab in complex with KIT D4D5 fragment was determined. Surface plasmon resonance analysis was employed to quantitatively characterize the kinetics and dissociation constants of the Fab (Kd = 6.4 pM). 3T3 cells expressing WT KIT receptor were incubated with the IgG and Fab versions of the antibody following stimulation with SCF. Lysates of unstimulated or SCF-stimulated cells were subjected to immunoprecipitation with the IgG and Fab followed by SDS/PAGE and immunoblotting with IgG and Fab. SCF-stimulated autophosphorylation of KIT was strongly inhibited upon binding of both fragments (5 nM for Fab and 1 nM for IgG). The IgG and Fab fragments inhibited the proliferation of KIT-Dependent Ba/F3 cells. The Fab fragment showed inhibition of cell growth at 5 nM, while the IgG fragment at 10 nM (Reshetnyak et al. 2013; PMID: 24127596).

Product Class:	Purified
Clone ID:	79D
Synonyms:	Mast/stem cell growth factor receptor Kit (EC:2.7.10.1); SCFR; Piebald trait protein; PBT; Proto-oncogene c-Kit; Tyrosine-protein kinase Kit; p145 c-kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; CD117
Amount:	200 μg
Application Codes Clone:	Crystallography; SPR; inhibition; IP; WB
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	Fab
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	The antibody is specific for KIT, and it binds to the membrane-proximal Ig-like D4 of KIT. The receptor tyrosine kinase KIT is a transmembrane protein that plays crucial roles in mediating diverse cellular processes, including cell differentiation, proliferation, and cell survival, among other activities.
Application Notes:	The crystal structure of the Fab in complex with KIT D4D5 fragment was determined. Surface plasmon resonance analysis was employed to quantitatively characterize the kinetics and dissociation constants of the Fab (Kd = 6.4 pM). 3T3 cells expressing WT KIT receptor were incubated with the IgG and Fab versions of the antibody following stimulation with SCF. Lysates of unstimulated or SCF-stimulated cells were subjected to immunoprecipitation with the IgG and Fab followed by SDS/PAGE and immunoblotting with IgG and Fab. SCF-stimulated autophosphorylation of KIT was strongly inhibited upon binding of both fragments (5 nM for Fab and 1 nM for IgG). The IgG and Fab fragments inhibited the proliferation of KIT-Dependent Ba/F3 cells. The Fab fragment showed inhibition of cell growth at 5 nM, while the IgG fragment at 10 nM (Reshetnyak et al. 2013; PMID: 24127596).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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