JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser.

Skip to the beginning of the images gallery

Mfg Part Number

Ab03170-1.1

Vector Labs Ab03170-1.1 Anti-E protein [Mab 4.2], Mouse IgG1, kappa (200 μg)

VECTOR LABS

Be the first to review this product

Quick Overview

Vector Labs Ab03170-1.1 Anti-E protein [Mab 4.2], Mouse IgG1, kappa (200 μg)

$517.40

Order will ship within 1-3 business days

Description

Product Class: Purified
Clone ID: Mab 4.2
Synonyms: tick-borne encephalitis virus; TBEV; LIV; Envelope protein Domain III
Amount: 200 μg
Application Codes Clone: ELISA; IF; Neutralizing; Crystallization; SPR; in vivo
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: scFv
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody binds to the lateral ridge of domain III of the E protein (EDIII) of LIV or TBEV, an epitope also reported for other potently neutralizing monoclonal antibodies against mosquito-borne flaviviruses (MBFVs), but adopts a unique binding orientation. Tick-borne encephalitis virus (TBEV) and louping ill virus (LIV) are members of the tick-borne flaviviruses (TBFVs) in the family Flaviviridae which cause encephalomeningitis and encephalitis in humans and other animals. The antibody reacts also with Negishi virus and Turkish sheep encephalomyelitis virus.
Application Notes: The antibody prevented mice against challenge with a lethal dose of LIV (Gao et al., 1994; PMID: 8126456). The specificity of the scFv fragment for LIV antigen was confirmed by ELISA analysis. Immunofluorescence was performed on PS cells infected with LIV or TBEV using the scFv and the original antibody. The antibody and the scFv fragment could neutralize both LIV and TBEV (Jiang et al., 1994; PMID: 8128623). The binding affinities of the scFv fragment for LIV E and for TBEV E were measured by surface plasmon resonance (respectively 45.4 nM and 390 nM for LIV and TBEV E). The structures of the scFv fragment in complex with the envelope protein from either TBEV or LIV were determined. The antibody showed potential as a therapeutic agent (Yang et al., 2019; PMID: 30760569).

Product Class:	Purified
Clone ID:	Mab 4.2
Synonyms:	tick-borne encephalitis virus; TBEV; LIV; Envelope protein Domain III
Amount:	200 μg
Application Codes Clone:	ELISA; IF; Neutralizing; Crystallization; SPR; in vivo
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	scFv
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody binds to the lateral ridge of domain III of the E protein (EDIII) of LIV or TBEV, an epitope also reported for other potently neutralizing monoclonal antibodies against mosquito-borne flaviviruses (MBFVs), but adopts a unique binding orientation. Tick-borne encephalitis virus (TBEV) and louping ill virus (LIV) are members of the tick-borne flaviviruses (TBFVs) in the family Flaviviridae which cause encephalomeningitis and encephalitis in humans and other animals. The antibody reacts also with Negishi virus and Turkish sheep encephalomyelitis virus.
Application Notes:	The antibody prevented mice against challenge with a lethal dose of LIV (Gao et al., 1994; PMID: 8126456). The specificity of the scFv fragment for LIV antigen was confirmed by ELISA analysis. Immunofluorescence was performed on PS cells infected with LIV or TBEV using the scFv and the original antibody. The antibody and the scFv fragment could neutralize both LIV and TBEV (Jiang et al., 1994; PMID: 8128623). The binding affinities of the scFv fragment for LIV E and for TBEV E were measured by surface plasmon resonance (respectively 45.4 nM and 390 nM for LIV and TBEV E). The structures of the scFv fragment in complex with the envelope protein from either TBEV or LIV were determined. The antibody showed potential as a therapeutic agent (Yang et al., 2019; PMID: 30760569).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

Reviews