Mfg Part Number
Ab03171-1.1
Vector Labs Ab03171-1.1 Anti-glycoprotein E [19/1786], Mouse IgG1, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing mice against the tick-borne encephalitis (TBE) virus, strain K23.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 19/1786 |
| Synonyms: | tick-borne encephalitis virus; TBEV; envelope domain III; EDIII; envelope protein |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; WB; IP; Cryo-EM; Neurtalization |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody is reported to bind amino acids 306-339 of the immunodominant loop structure of the glycoprotein E of tick-borne encephalitis virus. The antibody does not cross react with other flaviviruses. Tick-borne encephalitis virus (TBEV) is a member of the tick-borne flaviviruses (TBFVs) in the family Flaviviridae which cause encephalomeningitis and encephalitis in humans and other animals. |
| Application Notes: | The specificity of the antibody for glycoprotein E was confirmed by ELISA analysis. The antibody detected glycoprotein E by western blot analysis (Niedrig et al., 1994; PMID: 7817895). Neutralization of TBVE by the original format of the antibody and Fab fragment was determined, the EC50 values were respectively 0.24 ± 0.03 and 35.0 ± 2.5 µg ml−1. The cryo-EM structure of the Fab fragment in complex with Tick-borne encephalitis virus was determined. A fusion-from-without assay using C6/36 cells was performed to determine if the original format of the antibody and the Fab can prevent membrane fusion in vivo. Results showed that the native TBEV induced cell fusion at low pH, the virus in complex with the original format of the antibody lost this ability, and the virus in complex with the Fab induced cell fusion with lower efficiency than the native virus. The antibody has therapeutic potential (Fuzik et al. 2018; PMID: 29382836). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |