Vector Labs Ab03172-1.1 Anti-E protein [T025], Mouse IgG1, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: T025
Synonyms: tick-borne encephalitis virus; TBEV; envelope domain III; EDIII; envelope protein
Amount: 100 μg
Application Codes Clone: ELISA; Neutralizing; in vivo; Crystallization; IF
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for the envelope domain III (EDIII) of TBEV. It binds a conserved epitope near the lateral ridge of EDIII adjoining the EDI–EDIII hinge region of the E protein. The antibody recognises EDIII from all three TBEV subtypes: Western European (TBEVWE; e.g., Neudoerfl strain), Far Eastern (TBEVFE; e.g., Sofjin strain), and Siberian (TBEVSi; e.g., Vasilchenko strain). It also cross-reacts with EDIII from other tick-borne flaviviruses, including Langat (LGTV), louping ill (LIV), Omsk hemorrhagic fever (OHFV), Kyasanur forest disease (KFDV), and Powassan (POWV) viruses.
Application Notes: The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody showed neutralizing activity against TBEV RVPs (IC50 <1 ng/ml) and authentic TBEV Hypr strain (a TBEVWE isolate; IC50=67.2 ng/ml). Immunofluorescence was performed on PS cells using this antibody. Prophylaxis and therapeutic experiments in BALB/c mice infected with TBEV Hypr showed the antibody was efficacious in prevention (even at low doses ~0.005 mg/kg) and early treatment of the infection (up to 3 days post-infection). The crystal structure of the Fab fragment in complex with the EDIII domains from all three TBEV subtypes was determined (Agudelo et al. 2021; PMID: 33831141).

Product Class:	Purified
Clone ID:	T025
Synonyms:	tick-borne encephalitis virus; TBEV; envelope domain III; EDIII; envelope protein
Amount:	100 μg
Application Codes Clone:	ELISA; Neutralizing; in vivo; Crystallization; IF
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for the envelope domain III (EDIII) of TBEV. It binds a conserved epitope near the lateral ridge of EDIII adjoining the EDI–EDIII hinge region of the E protein. The antibody recognises EDIII from all three TBEV subtypes: Western European (TBEVWE; e.g., Neudoerfl strain), Far Eastern (TBEVFE; e.g., Sofjin strain), and Siberian (TBEVSi; e.g., Vasilchenko strain). It also cross-reacts with EDIII from other tick-borne flaviviruses, including Langat (LGTV), louping ill (LIV), Omsk hemorrhagic fever (OHFV), Kyasanur forest disease (KFDV), and Powassan (POWV) viruses.
Application Notes:	The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody showed neutralizing activity against TBEV RVPs (IC50 <1 ng/ml) and authentic TBEV Hypr strain (a TBEVWE isolate; IC50=67.2 ng/ml). Immunofluorescence was performed on PS cells using this antibody. Prophylaxis and therapeutic experiments in BALB/c mice infected with TBEV Hypr showed the antibody was efficacious in prevention (even at low doses ~0.005 mg/kg) and early treatment of the infection (up to 3 days post-infection). The crystal structure of the Fab fragment in complex with the EDIII domains from all three TBEV subtypes was determined (Agudelo et al. 2021; PMID: 33831141).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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