Mfg Part Number
Ab03175-4.0
Vector Labs Ab03175-4.0 Anti-Envelope protein domain III [POWV-80], Mouse IgG2c, kappa (200 μg)
Quick Overview
The original antibody was originated by immunizing C57BL/6J mice with POWV-SPO.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | POWV-80 |
| Synonyms: | Core protein; Envelope protein E; Matrix protein; Peptide pr; Protein pr; Small envelope protein M; POWV |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; Neutralizing; FC; Crystallography |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2c |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | The antibody binds to domain III of the envelope protein. The antibody cross reacts with tick-borne encephalitis virus (TBEV) and LGTV. |
| Application Notes: | The specificity of the antibody (IgG2c) was determined by ELISA. The antibody was used for flow cytometric analysis of HEK-293T cells. The antibody showed neutralizing activity on Vero cell incubated with POWV MA51240. The antibody showed neutralizing activity also against TBEV and LGTV (EC50 respectively 8.4 and 103 ng/ml). The binding affinity of the antibody to POWV E was measured using biolayer interferometry (BLI), showing the antibody binds POWV E with an affinity (KD) of ∼30 nM. The crystal structure of the Fab fragment in complex with POWV DIII was determined. The antibody might have therapeutic potential (VanBlargan et al., 2021; PMID: 33831142). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |