Mfg Part Number
Ab03176-10.0
Vector Labs Ab03176-10.0 Anti-Cytokeratin 5/6 [AbM58019-7H4], Human IgG1, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing 4-6 weeks old female ICR mice with recombinant protein comprising amino acids 488-590 of cytokeratin 5 in equal volume of Freund's incomplete adjuvant.
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| Product Class: | Purified |
|---|---|
| Clone ID: | AbM58019-7H4 |
| Synonyms: | CK5/6; CK5; CK-5; K5 KRT5; Keratin, type II cytoskeletal 5; cytokeratin-5; 58 kDa cytokeratin; Type-II keratin Kb5; CGMCC NO 21982; D5/16B4 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; IHC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | This antibody is capable of binding human cytokeratin 5 and human cytokeratin 6. Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. Cytokeratin 5/6 is often expressed in a basal cell layer of normal mammary ducts. Studies have also shown that CK5/6 can be used as a characteristic marker of basal-like breast cancer. |
| Application Notes: | The binding characterization of this antibody was done using ELISA. The original IgG2b antibody binds cytokeratin 5/6 with a high affinity constant of 3.84 × 10^(9) M^(-1). This antibody was also used in the immunohistochemical analysis of esophagus and esophageal squamous cell carcinoma. The immunohistochemical staining pattern of this antibody is similar to commercially available antibody D5/16B4 (CN113845592). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |