Mfg Part Number
Ab03180-23.0
Vector Labs Ab03180-23.0 Anti-Cytokeratin 14 [2G4], Rabbit IgG, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing 4-6 weeks old female ICR mice with recombinant protein comprising amino acids 458-472 of cytokeratin 7 in equal volume of Freund's incomplete adjuvant.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 2G4 |
| Synonyms: | CK14; CK-14; CK 14; K14; KRT14; keratin type I cytoskeletal 14; cytokeratin-14; keratin-14; CGMCC NO 20761; L1002 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; IHC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | This antibody is capable of binding human cytokeratin 14. Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. |
| Application Notes: | The binding characterization of this antibody was done using ELISA. The original IgG1 antibody binds cytokeratin 5/6 with a high affinity constant of 4.8× 10^(8) M^(-1). This antibody was also used in the immunohistochemical analysis of frozen paraffin sections of tonsil tissues and esophageal squamous cell carcinoma. The immunohistochemical staining pattern of this antibody is similar to commercially available antibody L1002 (CN113087793). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |