Mfg Part Number
Ab03190-2.0
Vector Labs Ab03190-2.0 Anti-Cytokeratin 20 [10A2], Mouse IgG2a, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing female ICR mice with recombinant human CK20 histidine-tagged fusion protein in Freund's complete adjuvant.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 10A2 |
| Synonyms: | CK20; CK-20; K20; KRT20; Cytokeratin-20; Keratin-20; Keratin type I cytoskeletal 20 ;Protein IT; CGMCC NO 20764; KS20.8 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; WB; IHC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody binds human cytokeratin 20. Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. There are 19 keratin polypeptides are found in human epithelium. Cytokeratin 20 plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, it plays a role in the secretion of mucin in the small intestine. |
| Application Notes: | The binding characterization of this antibody was done using ELISA. The affinity constant of the original IgG2a antibody was determined to be 1.92 × 10^9 M^(-1). This antibody was capable of detecting recombinant CK20 protein in a western blot assay. This antibody is similar to commercially available anti-Cytokeratin 20 antibody KS20.8 in staining various human tissues. This antibody was used in the immunohistochemical staining of stomach epithelial tissues and gastric adenocarcinomas (CN113583120). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |