Mfg Part Number
Ab03199-10.3
Vector Labs Ab03199-10.3 Anti-Glypican 3 [4C11], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing BLAB/c mice with recombinant N-terminal GPC3 antigen fragment emulsified in Freund's adjuvant.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 4C11 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | GPC3; Glypican-3; GPC-3; GTR2-2; MXR7; OCI5; DGSX; Intestinal protein OCI-5; CCTCC No: C2018156. |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; WB; IHC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | This antibody binds human glypican 3 (GPC3).Glypican-3 is an oncofetal antigen that belongs to the glypican family of glycosyl-phosphatidylinositol-anchored heparin sulfate proteoglycans. It negatively regulates the hedgehog signaling pathway and positively regulates the canonical Wnt signaling pathway. Multiple studies have revealed that GPC-3 is overexpressed in hepatocellular carcinoma (HCC). |
| Application Notes: | The initial binding characterization of this antibody was done using ELISA. This antibody can identify human glypican 3 expressed on the Huh7, LM3, HepG2, PLC, PVTT and SMMC-7721 (7721) cell lysates in a western blot assay. This antibody was also used in the immunohistochemical staining of human liver cancer tissues (CN111040036). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |