Vector Labs Ab03204-201.4 Anti-E Cadherin [19A11], Dog IgG2 (IgG-B), Fc Silent™, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 19A11
Heavy Chain Modification: Fc Silent™
Synonyms: CD324; Cadherin-1; Cadherin E; E-Cadherin; epithelial cadherin; CAM 120/80; Uvomorulin
Amount: 200 μg
Application Codes Clone: agonist; ELISA; WB; FC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric dog antibody was made using the variable domain sequences of the original Rat IgG2b format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody recognizes the N-terminal extracellular cadherin repeat domain 1 (EC1) of the human E-cadherin. This antibody also cross reacts with canine E-cadherin. Cadherins are calcium-dependent cell adhesion proteins. It is localized on the surfaces of epithelial cells in regions of cell-cell contact known as adherens junctions. E-cadherins are reported to play an important role in cancer metastasis.
Application Notes: The initial screeding of E-Cadherin–positive hybridoma supernatants for binding to live Colo 205 cells was done using an ELISA. Activating antibody 19A11 stimulated the adhesion of Colo 205 cells to pure E-cadherin substrate (PMID: 22513089). This antibody is also capable of recognizing denature human E-cadherin in a western blot. This antibody also inhibited the formation of epithelial tubules, causing the cells to remain as round epithelial cysts. This antibody also enhanced the adhesion of HGF-treated MDCK cells to E-cadherin when analyzed with the flow adhesion assay (PMID: 22513089). This antibody slowed the rate of wound closure in both normal culture medium and HGF-treated cultures (PMID: 22513089). This antibody is an E-cadherin activating antibody which stimulates the apoptosis of circulating tumor cells (PMID: 32127478). This antibody was used in determination of expression of E-cadherin on low-adherent Colo205 cells using flow cytometry (PMID: 27582386).

Product Class:	Purified
Clone ID:	19A11
Heavy Chain Modification:	Fc Silent™
Synonyms:	CD324; Cadherin-1; Cadherin E; E-Cadherin; epithelial cadherin; CAM 120/80; Uvomorulin
Amount:	200 μg
Application Codes Clone:	agonist; ELISA; WB; FC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric dog antibody was made using the variable domain sequences of the original Rat IgG2b format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody recognizes the N-terminal extracellular cadherin repeat domain 1 (EC1) of the human E-cadherin. This antibody also cross reacts with canine E-cadherin. Cadherins are calcium-dependent cell adhesion proteins. It is localized on the surfaces of epithelial cells in regions of cell-cell contact known as adherens junctions. E-cadherins are reported to play an important role in cancer metastasis.
Application Notes:	The initial screeding of E-Cadherin–positive hybridoma supernatants for binding to live Colo 205 cells was done using an ELISA. Activating antibody 19A11 stimulated the adhesion of Colo 205 cells to pure E-cadherin substrate (PMID: 22513089). This antibody is also capable of recognizing denature human E-cadherin in a western blot. This antibody also inhibited the formation of epithelial tubules, causing the cells to remain as round epithelial cysts. This antibody also enhanced the adhesion of HGF-treated MDCK cells to E-cadherin when analyzed with the flow adhesion assay (PMID: 22513089). This antibody slowed the rate of wound closure in both normal culture medium and HGF-treated cultures (PMID: 22513089). This antibody is an E-cadherin activating antibody which stimulates the apoptosis of circulating tumor cells (PMID: 32127478). This antibody was used in determination of expression of E-cadherin on low-adherent Colo205 cells using flow cytometry (PMID: 27582386).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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