Mfg Part Number
Ab03210-3.0
Vector Labs Ab03210-3.0 Anti-IL-13 [M1295], Mouse IgG2b, kappa (200 μg)
Quick Overview
The original mouse antibody m836 was generated by immunizing BALB/c mice with a natural variant of human IL-13 known as R130Q. The humanized version H2L6 was generated by grafting CDRs of mouse antibody onto selected human heavy chain and light chain frameworks and finally site-directed mutagenesis of two methionine in the CDRs of heavy chain with binding affinity optimization resulted in the generated of M1295.
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| Product Class: | Purified |
|---|---|
| Clone ID: | M1295 |
| Synonyms: | IL13; NC30; Interleukin-13; hC836; m836; H2L6; LC6HC2; HFAL62 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; neutralize; therapeutic |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | This antibody binds human interleukin-13 at the surface of helices A and D close to N- and C-termini of the IL-13 polypeptide chain. IL-13 is a cytokine secreted by T helper type 2 (Th2) cells, CD4 cells, natural killer T cell, mast cells, basophils, eosinophils and nuocytes. It inhibits the production of inflammatory cytokines (IL-1, IL-6, TNF, IL-8) by LPS-stimulated monocytes. It synergizes with IL2 in regulating interferon-gamma synthesis. It is a mediator of allergic inflammation and different diseases including asthma. |
| Application Notes: | This antibody can bind human IL13 in an ELISA. The affinity of original IgG1 antibody for wild type human IL-13 was determined by BIAcore to be 5.7 ± 1.3 pM (PMID: 20226193). Preclinical studies confirm the functional effects of M1295-mediated neutralization of human IL-13 in human cell based in vitro assays using mechanistically relevant endpoints associated with the pathogenesis of asthma, atopic dermatitis, and eosinophil-mediated disease (US8399630). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |