Mfg Part Number
Ab03216-10.3

Vector Labs Ab03216-10.3 Anti-IL-8 [6G4.2.5V11N35A], Human IgG1, Fc Silent™, kappa (200 μg)

Quick Overview
The original mouse antibody 6G4.2.5 was generated by immunizing BALB/c mice with recombinant human IL-8. The humanized version is 6G4.2.5 V11N35A was generated by grafting CDRs of mouse parental antibody onto human framework regions.
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Product Class:Purified
Clone ID:6G4.2.5V11N35A
Heavy Chain Modification:Fc Silent™
Synonyms:IL8; Interleukin-8; CXCL8; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; Granulocyte chemotactic protein 1; GCP-1; Monocyte-derived neutrophil chemotactic factor; MDNCF; Monocyte-derived neutrophil-activating peptide; MONAP; Neutrophil-activating protein 1; NAP-1; Protein 3-10C; T-cell chemotactic factor; 6G4.2.5; V11N35A; 6G4V11N35A
Amount:200 μg
Application Codes Clone:ELISA; neutralize
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG1
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:This antibody binds human IL-8, which is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. This antibody cross reacts with rabbit and rhesus monkey IL-8.
Application Notes:This antibody is capable of neutralizing the biological activity of wild-type human IL-8. This antibody is reported to inhibit human wild type IL-8, human monomeric IL-8, rabbit IL-8, and rhesus IL-8 mediated neutrophil chemotaxis (US6025158). The binding characterization of this antibody was done using ELISA. The Fab version of this antibody binds human IL-8 with an affinity of 114 pM (US6025158).
More Information
Brand Vector Labs
UNSPSC 12161500