Mfg Part Number
Ab03218-10.3
Vector Labs Ab03218-10.3 Anti-Prion protein [D18], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
PrP deficient (Prnp0/0) mice were immunized with disaggregated PrP 27-30 incorporated into liposomes. The original antibody was generated by panning by panning against dispersed SHaPrP 27-30.
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| Product Class: | Purified |
|---|---|
| Clone ID: | D18 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | PrPC; ASCR; PrP27-30; PrP33-35C; CD230 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; WB; inhibition; FC; IP; SPR; in vivo |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 18180775 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | Fab |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody recognises a discontinuous epitope and binds to residues 132-156 of PrP. |
| Application Notes: | The specificity of the antibody to recombinant SHaPrP(90-231) and with PrP 27-30 rods was confirmed by ELISA. The epitope mapping of this antibody was done using a paptide-based ELISA. The Fab fragment reacted with cell surface MoPrPc as showed by flow cytometry. The antibody efficiently immunoprecipitated SHaPrPC from transfected CHO cells (Williamson et al. 1998; PMID: 9765500). The ability of of the Fab fragment to inhibit prion propagation in a cultured mouse neuroblastoma cell line infected with PrPSc (ScN2a) was tested. The Fab fragment was found to abolish prion replication and to clear pre-exiting PrPSc, eliminating 50% of PrPSc from the cells within about 24 hours. Therefore, the antibody showed potential as a therapeutic agent. This antibody was used for detection of PrP with the surface of ScN2a cells by flow cytometry (Peretz et al., 2001; PMID: 11507642). The scFv version of the antibody was constructed and the specificity tested by ELISA. The binding affinity of the scFv fragment to PcP was measured by surface plasmon resonance (Kd= 10.1 nM). The scFv antibody gene was delivered to the brain by a recombinant adeno-associated virus, where it delayed the progression of the disease in a mouse model of TSE (Wuertzer et al., 2008; PMID: 18180775). The Fab fragment was conjugated to UltreSmall gold particles and used for immunolabelling for PrPC localization in mouse hippocampus (Mironov et al., 2003; PMID: 12904479). Immunofluorescence was performed on RK13 cells using this antibody (Spagnolli et al., 2021; PMID: 33437023). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |