Vector Labs Ab03218-23.0 Anti-Prion protein [D18], Rabbit IgG, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: D18
Synonyms: PrPC; ASCR; PrP27-30; PrP33-35C; CD230
Amount: 200 μg
Application Codes Clone: ELISA; WB; inhibition; FC; IP; SPR; in vivo
Shipping Temperature: Wet Ice
Origin Pub PMID: 18180775
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: Fab
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody recognises a discontinuous epitope and binds to residues 132-156 of PrP.
Application Notes: The specificity of the antibody to recombinant SHaPrP(90-231) and with PrP 27-30 rods was confirmed by ELISA. The epitope mapping of this antibody was done using a paptide-based ELISA. The Fab fragment reacted with cell surface MoPrPc as showed by flow cytometry. The antibody efficiently immunoprecipitated SHaPrPC from transfected CHO cells (Williamson et al. 1998; PMID: 9765500). The ability of of the Fab fragment to inhibit prion propagation in a cultured mouse neuroblastoma cell line infected with PrPSc (ScN2a) was tested. The Fab fragment was found to abolish prion replication and to clear pre-exiting PrPSc, eliminating 50% of PrPSc from the cells within about 24 hours. Therefore, the antibody showed potential as a therapeutic agent. This antibody was used for detection of PrP with the surface of ScN2a cells by flow cytometry (Peretz et al., 2001; PMID: 11507642). The scFv version of the antibody was constructed and the specificity tested by ELISA. The binding affinity of the scFv fragment to PcP was measured by surface plasmon resonance (Kd= 10.1 nM). The scFv antibody gene was delivered to the brain by a recombinant adeno-associated virus, where it delayed the progression of the disease in a mouse model of TSE (Wuertzer et al., 2008; PMID: 18180775). The Fab fragment was conjugated to UltreSmall gold particles and used for immunolabelling for PrPC localization in mouse hippocampus (Mironov et al., 2003; PMID: 12904479). Immunofluorescence was performed on RK13 cells using this antibody (Spagnolli et al., 2021; PMID: 33437023).

Product Class:	Purified
Clone ID:	D18
Synonyms:	PrPC; ASCR; PrP27-30; PrP33-35C; CD230
Amount:	200 μg
Application Codes Clone:	ELISA; WB; inhibition; FC; IP; SPR; in vivo
Shipping Temperature:	Wet Ice
Origin Pub PMID:	18180775
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	Fab
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody recognises a discontinuous epitope and binds to residues 132-156 of PrP.
Application Notes:	The specificity of the antibody to recombinant SHaPrP(90-231) and with PrP 27-30 rods was confirmed by ELISA. The epitope mapping of this antibody was done using a paptide-based ELISA. The Fab fragment reacted with cell surface MoPrPc as showed by flow cytometry. The antibody efficiently immunoprecipitated SHaPrPC from transfected CHO cells (Williamson et al. 1998; PMID: 9765500). The ability of of the Fab fragment to inhibit prion propagation in a cultured mouse neuroblastoma cell line infected with PrPSc (ScN2a) was tested. The Fab fragment was found to abolish prion replication and to clear pre-exiting PrPSc, eliminating 50% of PrPSc from the cells within about 24 hours. Therefore, the antibody showed potential as a therapeutic agent. This antibody was used for detection of PrP with the surface of ScN2a cells by flow cytometry (Peretz et al., 2001; PMID: 11507642). The scFv version of the antibody was constructed and the specificity tested by ELISA. The binding affinity of the scFv fragment to PcP was measured by surface plasmon resonance (Kd= 10.1 nM). The scFv antibody gene was delivered to the brain by a recombinant adeno-associated virus, where it delayed the progression of the disease in a mouse model of TSE (Wuertzer et al., 2008; PMID: 18180775). The Fab fragment was conjugated to UltreSmall gold particles and used for immunolabelling for PrPC localization in mouse hippocampus (Mironov et al., 2003; PMID: 12904479). Immunofluorescence was performed on RK13 cells using this antibody (Spagnolli et al., 2021; PMID: 33437023).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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