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Mfg Part Number

Ab03220-23.0

Vector Labs Ab03220-23.0 Anti-Lysozyme [F9.13.7], Rabbit IgG, kappa (200 μg)

VECTOR LABS

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Quick Overview

The antibody was raised against hen egg-white lysozyme.

$517.40

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Description

Product Class: Purified
Clone ID: F9.13.7
Synonyms: Lysozyme C; 1,4-beta-N-acetylmuramidase C; GEL; hen egg-white lysozyme
Amount: 200 μg
Application Codes Clone: ELISA; Crystallography
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: The antibody binds to lysozyme, in particular to residues H15, G16, Y20, R21, W63, R73, L75, N77, N93, K96, K97, S100, D101. The antibody partially overlaps the epitope recognized by antibody HyHEL10. Lysozyme degrades polysacchardies of various types of bacterial cell walls.
Application Notes: The specificity of the original format of the antibody was confirmed by ELISA analysis (Lescar et al., 1993; PMID: 7680133). The structure of the Fab fragment in complex with Guinea-Fowl Lisozyme was determined (Lescar et al., 1995; PMID: 7629116). Homogeneous kinetics of Fab and Fv fragments were studied and the KD values were 0.57 ± 0.05 nM for scFv and 1.6 ± 0.2 nM for Fab. Since the clone and HyHEL-10 recognized a common epitope, equilibria and kinetics of the two clones were evaluated. Results showed that the clone had greater specificity than HyHEL-10 despite its lower affinity (Pons et al., 2002; PMID: 12237453). The ability of the antibody to modulate in vitro the presentation of hen egg-white lysozyme (HEL) to specific T cells was evaluated, results showed the antibody was able to enhance the HEL presentation to L3E10 T cells (Guermonprez et al., 1999).

Product Class:	Purified
Clone ID:	F9.13.7
Synonyms:	Lysozyme C; 1,4-beta-N-acetylmuramidase C; GEL; hen egg-white lysozyme
Amount:	200 μg
Application Codes Clone:	ELISA; Crystallography
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	The antibody binds to lysozyme, in particular to residues H15, G16, Y20, R21, W63, R73, L75, N77, N93, K96, K97, S100, D101. The antibody partially overlaps the epitope recognized by antibody HyHEL10. Lysozyme degrades polysacchardies of various types of bacterial cell walls.
Application Notes:	The specificity of the original format of the antibody was confirmed by ELISA analysis (Lescar et al., 1993; PMID: 7680133). The structure of the Fab fragment in complex with Guinea-Fowl Lisozyme was determined (Lescar et al., 1995; PMID: 7629116). Homogeneous kinetics of Fab and Fv fragments were studied and the KD values were 0.57 ± 0.05 nM for scFv and 1.6 ± 0.2 nM for Fab. Since the clone and HyHEL-10 recognized a common epitope, equilibria and kinetics of the two clones were evaluated. Results showed that the clone had greater specificity than HyHEL-10 despite its lower affinity (Pons et al., 2002; PMID: 12237453). The ability of the antibody to modulate in vitro the presentation of hen egg-white lysozyme (HEL) to specific T cells was evaluated, results showed the antibody was able to enhance the HEL presentation to L3E10 T cells (Guermonprez et al., 1999).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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