Vector Labs Ab03224-1.159-BS Anti-3-Phenoxybenzoic acid [3P5ThC12], Mouse IgG1-Fc Fusion, (1 mg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 3P5ThC12
Synonyms: 3-PBA; 3739-38-6; m-Phenoxybenzoic acid; 3-Carboxydiphenyl ether
Amount: 1 mg
Application Codes Clone: ELISA
Shipping Temperature: Wet Ice
Buffer Composition: PBS only.
Original Format: VHH
Storage Temperature: Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: The antibody is highly specific and sensitive for 3-PBA and its 4-hydroxylated derivative, 4-hydroxy 3-PBA. The antibody does not cross reacts with 3-Phenoxybenzyl aldehyde, 3-PBA-glycine conjugate, 3-Phenoxybenzyl alcohol, Permethrin, Cypermethrin, Esfenvalerate, Deltamethrin, Cyfluthurin. 3-PBA is a human urinary metabolite of pyrethroid insecticides and can be used as a biomarker to monitor human exposure to these pesticides.
Application Notes: The VHH fragment was employed in an indirect competitive VELISA against 3-PBA, showing an IC50 = 1.4 ng/ml. The performance of the VHH fragment was also evaluated using PELISA, the IC50 value for the VHH was 0.6 ng/ml. Both assays showed a similar tolerance to methanol and dimethylsulfoxide up to 50% in assay buffer. The VELISA and PELISA were used for the immunodetection of 3-PBA in human urine samples (Kim et al., 2012; PMID: 22148739). The VHH fragment was used to construct a VHH-alkaline phosphatase (AP) fusion protein. The fusion protein was used to develop a direct competitive fluorescence enzyme immunoassay for detection of 3-PBA in urine. The cd-FEIA showed 50% inhibitory concentration (IC50) and the linear range of respectively 0.082 and 0.015-0.447 ng/ml, and a detection limit of 0.011 ng/ml (Huo et al., 2018; PMID: 30293433). The VHH fragment was employed to develop a label-free, optical whole-cell Escherichia coli biosensor for the detection of 3-PBA, showing a limit of detection of 3ng/ml (Riangrungro et al. 2019; PMID: 31462650).

Product Class:	Purified
Clone ID:	3P5ThC12
Synonyms:	3-PBA; 3739-38-6; m-Phenoxybenzoic acid; 3-Carboxydiphenyl ether
Amount:	1 mg
Application Codes Clone:	ELISA
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS only.
Original Format:	VHH
Storage Temperature:	Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	The antibody is highly specific and sensitive for 3-PBA and its 4-hydroxylated derivative, 4-hydroxy 3-PBA. The antibody does not cross reacts with 3-Phenoxybenzyl aldehyde, 3-PBA-glycine conjugate, 3-Phenoxybenzyl alcohol, Permethrin, Cypermethrin, Esfenvalerate, Deltamethrin, Cyfluthurin. 3-PBA is a human urinary metabolite of pyrethroid insecticides and can be used as a biomarker to monitor human exposure to these pesticides.
Application Notes:	The VHH fragment was employed in an indirect competitive VELISA against 3-PBA, showing an IC50 = 1.4 ng/ml. The performance of the VHH fragment was also evaluated using PELISA, the IC50 value for the VHH was 0.6 ng/ml. Both assays showed a similar tolerance to methanol and dimethylsulfoxide up to 50% in assay buffer. The VELISA and PELISA were used for the immunodetection of 3-PBA in human urine samples (Kim et al., 2012; PMID: 22148739). The VHH fragment was used to construct a VHH-alkaline phosphatase (AP) fusion protein. The fusion protein was used to develop a direct competitive fluorescence enzyme immunoassay for detection of 3-PBA in urine. The cd-FEIA showed 50% inhibitory concentration (IC50) and the linear range of respectively 0.082 and 0.015-0.447 ng/ml, and a detection limit of 0.011 ng/ml (Huo et al., 2018; PMID: 30293433). The VHH fragment was employed to develop a label-free, optical whole-cell Escherichia coli biosensor for the detection of 3-PBA, showing a limit of detection of 3ng/ml (Riangrungro et al. 2019; PMID: 31462650).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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