Mfg Part Number
Ab03225-23.0
Vector Labs Ab03225-23.0 Anti-Cry1 [3H9], Rabbit IgG, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing Balb/c mice with a mixture of six Cry1 toxins (Cry1Aa, Cry1Ab, Cry1Ac, Cry1B, Cry1C, and Cry1F) followed by antibody library construction and phage display-based selection.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 3H9 |
| Synonyms: | Cry1 toxins; Cry1Aa; Cry1Ab; Cry1Ac; Cry1B; Cry1C; Cry1F; Pesticidal crystal protein Cry1Aa; 133 kDa crystal protein; Crystaline entomocidal protoxin; Insecticidal delta-endotoxin CryIA(a); Pesticidal crystal protein Cry1Fa; 134 kDa crystal protein; Insecticidal delta-endotoxin CryIF(a); Pesticidal crystal protein Cry1Ab; 130 kDa crystal protein; Insecticidal delta-endotoxin CryIA(b); Pesticidal crystal protein Cry1Ac;133 kDa crystal protein; Insecticidal delta-endotoxin CryIA(c) |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length chimeric rabbit antibody was made using the variable domain sequences of the original Mouse scFv format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | The antibody binds to Cry1 toxins (Cry1Aa, Cry1Ab, Cry1Ac, Cry1B, Cry1C, Cry1F). The antibody does not cross react with Cry2A and vip3. |
| Application Notes: | The specificity of the scFv antibody for the six Cry1 toxins was confirmed by ELISA. The scFv fragment was used to establish the double antibody sandwich enzyme-linked immunosorbent assay method (DAS-ELISA) for detecting six Cry1 toxins (Cry1Aa, Cry1Ab, Cry1Ac, Cry1B, Cry1C, and Cry1F). The lowest detectable limits (LOD) and the lowest quantitative limits (LOQ) were 3.14–11.07 and 8.22–39.44 ng mL−1 , respectively, with the correlation coefficient higher than 0.997. The average recoveries of Cry1 toxins from spiked rice leaf samples were ranged from 84 to 95%, with coefficient of variation (CV) less than 8.2%, showing good accuracy for the multi-residue determination of six Cry1 toxins in agricultural samples (Dong et al., 2018; PMID: 29484477). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |