Mfg Part Number
Ab03227-1.1
Vector Labs Ab03227-1.1 Anti-Histone H4 [1A9D7], Mouse IgG1, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing mice with Histone H4 protein with site-specific acetylations at K5 and K8.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 1A9D7 |
| Synonyms: | H4K5acK8ac; Histone H4 protein; |
| Amount: | 200 μg |
| Application Codes Clone: | Crystallization; ELISA; WB |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | The antibody specifically recognizes simultaneous acetylations at K5 and K8 of histone H4. Lysine acetylation of core histones is one of the major posttranslational modifications involved in the control of eukaryotic gene expression. |
| Application Notes: | The specificity of the original format of the antibody for histone H4 proteins containing di-acetylation of K5acK8ac was confirmed by ELISA analysis. The antibody detected the histone H4 proteins containing at least the di-acetylation of K5acK8ac western blot analysis. The crystal structure of the complex of di-acetylated histone H4 and 1A9D7 Fab fragment was determined (Handoko et al., 2018; PMID: 30080437). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |