Mfg Part Number
Ab03229-23.0
Vector Labs Ab03229-23.0 Anti-FAP [73.3], Rabbit IgG, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing FAP-null mice with FAP-expressing 3T3 cells.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 73.3 |
| Synonyms: | Fibroblast activation protein; Fibroblast activation protein-α; Prolyl endopeptidase FAP; Dipeptidyl peptidase FAP; Fibroblast activation protein alpha; FAPalpha1; Gelatine degradation protease FAP; Integral membrane serine protease; Post-proline cleaving enzyme;Serine integral membrane protease; SIMP; Surface-expressed protease; Seprase |
| Amount: | 200 μg |
| Application Codes Clone: | WB; IP; FC; in vitro; in vivo |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 24778279 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | The antibody is specific for fibroblast activation protein (FAP). FAP is a membrane protease that is highly expressed by cancer-associated fibroblasts (CAFs). |
| Application Notes: | A second-generation retroviral CAR, consisting of the scFv fragment of the antibody, with CD8 stalk, human CD3ζ and 4–1BB domains, targeting mouse FAP was developed. The Fap-CAR-T cells killed FAP-expressing 3T3 target cells specifically. The FAP-CAR-T cells were tested in vivo in three different established models, showing that the growth of tumors was significantly reduced by 35–50% following treatment. Further, the anti-FAP-CAR T cells demonstrated enhanced antitumor response when combined with a tumor vaccine. No significant toxicity was observed in the treated mice. (Wang et al., 2014; PMID: 24778279) FAP+ cells were identified using flow cytometry of lung tissue using the antibody (Kimura et al., 2019; PMID: 31188013). This antibody was used for detection of murine FAP expression on HT1080 cells by flow cytometry (de Sostoa et al. 2019, PMID: 30683154). Immunoprecipitation of FAP was performed on lung humogenate using this antibody. The antibody detected FAP by western blot analysis (Fan et al., 2016; PMID: 26663085). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |