Mfg Part Number
Ab03235-1.159-BS

Vector Labs Ab03235-1.159-BS Anti-CTLA-4 [NB25B-17], Mouse IgG1-Fc Fusion, (1 mg)

Quick Overview
The original antibody was generated by immunizing alpaca with human CTLA-4, followed by antibody library construction and phage display-based selection.
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Product Class:Purified
Clone ID:NB25B-17
Synonyms:Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; CD152
Amount:1 mg
Application Codes Clone:FACS; in vivo
Shipping Temperature:Wet Ice
Buffer Composition:PBS only.
Original Format:VHH
Storage Temperature:Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:The antibody is specific for CTLA-4. CTLA-4 is a protein receptor that functions as an immune checkpoint and downregulates immune responses.
Application Notes:The binding affinity of humanized VHH-Fc chimeric antibody to human CTLA-4 expressed in CHO cells was shown by FACS experiments. The blocking activity of the humanized VHH-Fc chimeric antibody to the binding of CTLA-4 to CD80 or CD86 was shown using FACS. The antitumor activity of the humanized antibody was demonstrated in vivo in animal models. Specifically, a tumor animal model was established by inoculating mice with human breast cancer cells MDA-MB-231 (ATCC) to induce breast cancer. Mice were grouped and administered with the humanized version of the antibody, and tumor volume in tumor-bearing animals was measured periodically in size. Results showed that the humanized version of the antibody was able to significantly reduce tumor volume in tumor-bearing mice (CN113121689).
More Information
Brand Vector Labs
UNSPSC 12161500