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Mfg Part Number

Ab03244-23.0

Vector Labs Ab03244-23.0 Anti-CENP-B [5D1], Rabbit IgG, lambda (200 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by immunizing a barnevelder chicken with an antibody antigen cocktail comprising recombinant purified human antigens annexin XI, CENP-B, Topo, Jo-1, Ro60, Rpp38 and U1A in complete Freund's adjuvant. B cells that were harvested from the bone marrow and peripheral blood lymphocytes of immunized chickens was used to generate recombinant scFv phage display libraries. The specific clone was identified by panning the library against specific antigen.

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Description

Product Class: Purified
Clone ID: 5D1
Synonyms: CENPB; Centromere protein B; Major centromere autoantigen B
Amount: 200 μg
Application Codes Clone: ELISA; WB
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: scFv
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This full-length chimeric rabbit antibody was made using the variable domain sequences of the original Chicken scFv format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody binds human major centromere autoantigen B (CENP-B). It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. It interacts with centromeric heterochromatin in chromosomes and binds to a specific 17 bp subset of alphoid satellite DNA, called the CENP-B box. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.
Application Notes: The binding characterization of this antibody was done using ELISA. This antibody is also capable of recognizing human CENP-B in a western blot assay (PMID: 18062792).

Product Class:	Purified
Clone ID:	5D1
Synonyms:	CENPB; Centromere protein B; Major centromere autoantigen B
Amount:	200 μg
Application Codes Clone:	ELISA; WB
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	scFv
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This full-length chimeric rabbit antibody was made using the variable domain sequences of the original Chicken scFv format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody binds human major centromere autoantigen B (CENP-B). It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. It interacts with centromeric heterochromatin in chromosomes and binds to a specific 17 bp subset of alphoid satellite DNA, called the CENP-B box. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies.
Application Notes:	The binding characterization of this antibody was done using ELISA. This antibody is also capable of recognizing human CENP-B in a western blot assay (PMID: 18062792).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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