Mfg Part Number
Ab03245-30.11
Vector Labs Ab03245-30.11 Anti-DNA topoisomerase 1 [5C2], scFv fragment (His), ScFv, (50 μg)
Quick Overview
The original antibody was generated by immunizing a barnevelder chicken with an antibody antigen cocktail comprising recombinant purified human antigens annexin XI, CENP-B, Topo, Jo-1, Ro60, Rpp38 and U1A in complete Freund's adjuvant. B cells that were harvested from the bone marrow and peripheral blood lymphocytes of immunized chickens was used to generate recombinant scFv phage display libraries. The specific clone was identified by panning the library against specific antigen.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 5C2 |
| Heavy Chain Modification: | ScFv |
| Synonyms: | TOP1; Topo; DNA topoisomerase I |
| Amount: | 50 μg |
| Application Codes Clone: | ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody binds human topoisomerase 1. This enzyme controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. |
| Application Notes: | The binding characterization of this antibody was done using ELISA (PMID: 18062792). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |