Vector Labs Ab03251-10.0 Anti-Mesothelin [MN-1], Human IgG1, kappa (50 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: MN-1
Synonyms: MSLN; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor; MN
Amount: 50 μg
Application Codes Clone: ELISA; SPR; FC; WB; IHC; IP
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2a
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody can recognize and bind human mesothelin. This antibody binds a different antibody on the human mesothelin than another antibody called MB. Mesothelin (MSLN) is a glycophosphatidylinositol (GPI)-linked cell surface protein highly expressed in several types of malignant tumors sometimes in association with increased tumor aggressiveness and poor clinical outcome. The membrane-anchored forms of this molecule may play a role in cellular adhesion.
Application Notes: The binding characterization of this antibody was done using ELISA and SPR. The binding affinity of the original IgG2a antibody for mesothelin-Fc protein was determined by BIACore and reported to be Kd = 1.0 nM. This antibody can recognize membrane bound mesothelin on the surface of mesothelin positive H226, A1847, Hela and Panc3.014 cells (mesothelin-positive) in a flow cytometry. This antibody can also bind denatured human mesothelin in a western blot assay. This antibody was also used in the immunohistochemical staining of cancerous tissue like ovarian adenocarcinoma and normal tissues like peritoneal mesothelium on uterus, pleural mesothelium on lung and fallopian tube luminal fluid (PMID: 16115924). This antibody was also used in an immunohistochemical analysis of 1562 tumors for mesothelin expression. It was reported that mesothelin was highly expressed in ovarian serous carcinoma, pancreatic adenocarcinoma and malignant pleural mesothelioma (PMID: 28460459). This antibody was also used in the immunoprecipitation of mesothelin from cell lysates (PMID: 23694968).

Product Class:	Purified
Clone ID:	MN-1
Synonyms:	MSLN; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor; MN
Amount:	50 μg
Application Codes Clone:	ELISA; SPR; FC; WB; IHC; IP
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2a
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody can recognize and bind human mesothelin. This antibody binds a different antibody on the human mesothelin than another antibody called MB. Mesothelin (MSLN) is a glycophosphatidylinositol (GPI)-linked cell surface protein highly expressed in several types of malignant tumors sometimes in association with increased tumor aggressiveness and poor clinical outcome. The membrane-anchored forms of this molecule may play a role in cellular adhesion.
Application Notes:	The binding characterization of this antibody was done using ELISA and SPR. The binding affinity of the original IgG2a antibody for mesothelin-Fc protein was determined by BIACore and reported to be Kd = 1.0 nM. This antibody can recognize membrane bound mesothelin on the surface of mesothelin positive H226, A1847, Hela and Panc3.014 cells (mesothelin-positive) in a flow cytometry. This antibody can also bind denatured human mesothelin in a western blot assay. This antibody was also used in the immunohistochemical staining of cancerous tissue like ovarian adenocarcinoma and normal tissues like peritoneal mesothelium on uterus, pleural mesothelium on lung and fallopian tube luminal fluid (PMID: 16115924). This antibody was also used in an immunohistochemical analysis of 1562 tumors for mesothelin expression. It was reported that mesothelin was highly expressed in ovarian serous carcinoma, pancreatic adenocarcinoma and malignant pleural mesothelioma (PMID: 28460459). This antibody was also used in the immunoprecipitation of mesothelin from cell lysates (PMID: 23694968).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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