Vector Labs Ab03255-23.0 Anti-Nicotine [NIC9D9], Rabbit IgG, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: NIC9D9
Synonyms: Nicotin; rac-3-(1-methylpyrrolidin-2-yl)pyridine; CAS: 54-11-5; CHEBI:18723; Nic12; 9D9
Amount: 200 μg
Application Codes Clone: ELISA; immunotherapy
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody is highly specific for S-(–)-nicotine. Nicotine is a plant alkaloid, found in the tobacco plant and other plants of the nightshade family. It is an addictive central nervous system (CNS) stimulant that causes either ganglionic stimulation in low doses or ganglionic blockage in high doses. It is the main psychoactive ingredient in tobacco products and a major component of cigarettes, which is also is used therapeutically to help with smoking cessation and reduce withdrawal symptoms.
Application Notes: The binding characterization of this antibody to nicotine-BSA hapten was done using ELISA (PMID: 11397142). A study to determine the effect of vaccination with this antibody towards a series of nicotine challenges and testing sessions was conducted. Passive immunization with NIC9D9 resulted in a 66.9% decrease in locomotor activity versus a 3.4% decrease in controls (PMID: 14738965). This antibody was also used in the generation of an adeno-associated virus (AAV) gene transfer vector which persistently expresses this antibody in vivo and prevents nicotine from reaching its receptors in the brain. This vector is called AAVantiNic and it generates an antibody similar to Nic9D9 that binds nicotine with an affinity of Kd= 43 nM. The mice treated with this vector showed 7 times greater amounts of nicotine in the serum of which 83% was bound to IgG. The vector blocked nicotine-mediated alterations in arterial blood pressure, heart rate and locomotor activity (PMID: 22745437).

Product Class:	Purified
Clone ID:	NIC9D9
Synonyms:	Nicotin; rac-3-(1-methylpyrrolidin-2-yl)pyridine; CAS: 54-11-5; CHEBI:18723; Nic12; 9D9
Amount:	200 μg
Application Codes Clone:	ELISA; immunotherapy
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody is highly specific for S-(–)-nicotine. Nicotine is a plant alkaloid, found in the tobacco plant and other plants of the nightshade family. It is an addictive central nervous system (CNS) stimulant that causes either ganglionic stimulation in low doses or ganglionic blockage in high doses. It is the main psychoactive ingredient in tobacco products and a major component of cigarettes, which is also is used therapeutically to help with smoking cessation and reduce withdrawal symptoms.
Application Notes:	The binding characterization of this antibody to nicotine-BSA hapten was done using ELISA (PMID: 11397142). A study to determine the effect of vaccination with this antibody towards a series of nicotine challenges and testing sessions was conducted. Passive immunization with NIC9D9 resulted in a 66.9% decrease in locomotor activity versus a 3.4% decrease in controls (PMID: 14738965). This antibody was also used in the generation of an adeno-associated virus (AAV) gene transfer vector which persistently expresses this antibody in vivo and prevents nicotine from reaching its receptors in the brain. This vector is called AAVantiNic and it generates an antibody similar to Nic9D9 that binds nicotine with an affinity of Kd= 43 nM. The mice treated with this vector showed 7 times greater amounts of nicotine in the serum of which 83% was bound to IgG. The vector blocked nicotine-mediated alterations in arterial blood pressure, heart rate and locomotor activity (PMID: 22745437).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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