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Mfg Part Number

Ab03256-1.159

Vector Labs Ab03256-1.159 Anti-Caffeine [VSA2], Mouse IgG1-Fc Fusion, (100 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by immunizing camelid species (llama and camel)) with caffeine-KLH conjugate. The peripheral blood lymphocytes from the camels was used to generate VHH phage display libraries and the particular clones was isolated by screening the library against caffeine-BSA conjugate.

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Description

Product Class: Purified
Clone ID: VSA2
Synonyms: C8H10N4O2; guaranine; methyltheobromine; 1 3 7-Trimethylxanthine; 7-methyltheophylline; theine theophylline; theobromine; paraxanthine; CHEBI:27732; α-Caff-A
Amount: 100 μg
Application Codes Clone: ELISA
Shipping Temperature: Wet Ice
Origin Pub PMID: 19560409
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: VHH
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody binds caffeine and has low affinity for compounds like theophylline, theobromine and paraxanthine. Caffeine is a central nervous system (CNS) stimulant of the methylxanthine class. The best-known source of caffeine is the coffee bean, the seed of the Coffea plant. It is present in drinks like coffee, tea and colas.
Application Notes: This antibody is highly heat stable and is shown to recover its reactivity after exposure to temperatures up to 90 degrees C. This antibody was able to bind caffeine at 70 degrees C. The binding reactivity of this antibody for caffeine and other compounds like theophylline, theobromine and paraxanthine was determined using ELISA (PMID: 16808459; 19560409; US7615218). A competition caffeine ELISA was developed for the measurement of caffeine content in beverages like coffee, Coca-Cola and Diet Coke. This antibody is capable of removing caffeine from coffee and furthermore can remove caffeine from even “decaffeinated” coffee (PMID: 16808459; US7615218).

Product Class:	Purified
Clone ID:	VSA2
Synonyms:	C8H10N4O2; guaranine; methyltheobromine; 1 3 7-Trimethylxanthine; 7-methyltheophylline; theine theophylline; theobromine; paraxanthine; CHEBI:27732; α-Caff-A
Amount:	100 μg
Application Codes Clone:	ELISA
Shipping Temperature:	Wet Ice
Origin Pub PMID:	19560409
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	VHH
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody binds caffeine and has low affinity for compounds like theophylline, theobromine and paraxanthine. Caffeine is a central nervous system (CNS) stimulant of the methylxanthine class. The best-known source of caffeine is the coffee bean, the seed of the Coffea plant. It is present in drinks like coffee, tea and colas.
Application Notes:	This antibody is highly heat stable and is shown to recover its reactivity after exposure to temperatures up to 90 degrees C. This antibody was able to bind caffeine at 70 degrees C. The binding reactivity of this antibody for caffeine and other compounds like theophylline, theobromine and paraxanthine was determined using ELISA (PMID: 16808459; 19560409; US7615218). A competition caffeine ELISA was developed for the measurement of caffeine content in beverages like coffee, Coca-Cola and Diet Coke. This antibody is capable of removing caffeine from coffee and furthermore can remove caffeine from even “decaffeinated” coffee (PMID: 16808459; US7615218).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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