Mfg Part Number
Ab03256-10.159-BS

Vector Labs Ab03256-10.159-BS Anti-Caffeine [VSA2], Human IgG1-Fc Fusion, (1 mg)

Quick Overview
The original antibody was generated by immunizing camelid species (llama and camel)) with caffeine-KLH conjugate. The peripheral blood lymphocytes from the camels was used to generate VHH phage display libraries and the particular clones was isolated by screening the library against caffeine-BSA conjugate.
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Product Class:Purified
Clone ID:VSA2
Synonyms:C8H10N4O2; guaranine; methyltheobromine; 1 3 7-Trimethylxanthine; 7-methyltheophylline; theine theophylline; theobromine; paraxanthine; CHEBI:27732; α-Caff-A
Amount:1 mg
Application Codes Clone:ELISA
Shipping Temperature:Wet Ice
Origin Pub PMID:19560409
Buffer Composition:PBS only.
Original Format:VHH
Storage Temperature:Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:This antibody binds caffeine and has low affinity for compounds like theophylline, theobromine and paraxanthine. Caffeine is a central nervous system (CNS) stimulant of the methylxanthine class. The best-known source of caffeine is the coffee bean, the seed of the Coffea plant. It is present in drinks like coffee, tea and colas.
Application Notes:This antibody is highly heat stable and is shown to recover its reactivity after exposure to temperatures up to 90 degrees C. This antibody was able to bind caffeine at 70 degrees C. The binding reactivity of this antibody for caffeine and other compounds like theophylline, theobromine and paraxanthine was determined using ELISA (PMID: 16808459; 19560409; US7615218). A competition caffeine ELISA was developed for the measurement of caffeine content in beverages like coffee, Coca-Cola and Diet Coke. This antibody is capable of removing caffeine from coffee and furthermore can remove caffeine from even “decaffeinated” coffee (PMID: 16808459; US7615218).
More Information
Brand Vector Labs
UNSPSC 12161500