Mfg Part Number
Ab03261-3.3
Vector Labs Ab03261-3.3 Anti-Pyrrolate Protein [3C8], Mouse IgG2b, Fc Silent™, kappa (200 μg)
Quick Overview
The original antibody was isolated from the repertoire of the B-1a cell populations in the apoE deficient (apoE−/−) mice. Spleen cells from male apoE−/− mice at 21 weeks of age were fused with P3/U1 murine myeloma cells, and after screening based on specific binding to pyrBSA
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | 3C8 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | pyrP; Lysine N-pyrrolation; pyrK |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 35031322 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgM |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgM format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | This antibody reacts specifically with pyrrolated protein and does not cross react with DNA. Lysine N-pyrrolation is a post translational modification, a unique covalent modification that converts lysine residues to Nε-pyrrole-L-lysine (pyrK). It imparts electronegative properties to proteins, causing them to mimic DNA and the proteins acquire an electrical conductivity via lysine N-pyrrolation. |
| Application Notes: | This antibody is recommended for determination of pyrrolated proteins in a sample. The binding reactivity of this antibody towards pyrrolated-BSA protein was determined using ELISA. This antibody does no cross react with DNA in ELISA (PMID: 35031322). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |