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Mfg Part Number

Ab03275-10.3

Vector Labs Ab03275-10.3 Anti-EGFR [cet.Hum (Cetuximab - humanized; hC225)], Human IgG1, Fc Silent™, kappa (200 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by grafting CDR loops of cetuximab on framework regions derived from type 3 (VH3 and VL3 kappa) human germline sequences to obtain recombinant VH and VL domains linked together with a flexible linker [(Gly4Ser)3] to form a scFv.

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Description

Product Class: Purified
Clone ID: cet.Hum (Cetuximab - humanized; hC225)
Heavy Chain Modification: Fc Silent™
Synonyms: ERBB1; ERBB; ErbB-1, Erb-B1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
Amount: 200 μg
Application Codes Clone: ELISA; WB; FC; block
Shipping Temperature: Wet Ice
Origin Pub PMID: 29185855
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: scFv
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This full-length reformatted human antibody was made using the variable domain sequences of the original Human scFv format for improved compatibility with existing reagents assays and techniques.
Specificity Statement: This antibody recognizes human EGFR. The epidermal growth factor receptor is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands.
Application Notes: The binding characterization of this humanized scFv version of cetuximab to human EGFR was done using ELISA. This antibody can recognize human EGFR from lysates of A-431 and MCF-7 cells in western blot. The scFv version was able to recognize EGFR on over-expressing cancer cells (A-431) but failed to detect cancer cells with low levels of EGFR (MCF-7 cells) in a flow cytometric experiment. The binding affinity of this scFv version was reported to be 9 fold lower than that of the parental cetuximab, but still it was strong enough for the successful recognition of human EGFR expressing cells. This antibody can be used to block cancer cell surface receptors and inhibit their downstream signaling (PMID: 29185855).

Product Class:	Purified
Clone ID:	cet.Hum (Cetuximab - humanized; hC225)
Heavy Chain Modification:	Fc Silent™
Synonyms:	ERBB1; ERBB; ErbB-1, Erb-B1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
Amount:	200 μg
Application Codes Clone:	ELISA; WB; FC; block
Shipping Temperature:	Wet Ice
Origin Pub PMID:	29185855
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	scFv
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This full-length reformatted human antibody was made using the variable domain sequences of the original Human scFv format for improved compatibility with existing reagents assays and techniques.
Specificity Statement:	This antibody recognizes human EGFR. The epidermal growth factor receptor is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands.
Application Notes:	The binding characterization of this humanized scFv version of cetuximab to human EGFR was done using ELISA. This antibody can recognize human EGFR from lysates of A-431 and MCF-7 cells in western blot. The scFv version was able to recognize EGFR on over-expressing cancer cells (A-431) but failed to detect cancer cells with low levels of EGFR (MCF-7 cells) in a flow cytometric experiment. The binding affinity of this scFv version was reported to be 9 fold lower than that of the parental cetuximab, but still it was strong enough for the successful recognition of human EGFR expressing cells. This antibody can be used to block cancer cell surface receptors and inhibit their downstream signaling (PMID: 29185855).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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