Vector Labs Ab03291-2.3 Anti-Collagen type II [CIIC1], Mouse IgG2a, Fc Silent™, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: CIIC1
Heavy Chain Modification: Fc Silent™
Synonyms: CII; Alpha-1 type II collagen; Collagen alpha-1(II) chain
Amount: 200 μg
Application Codes Clone: ELISA; Crystallization; in vivo; WB; in vitro; IHC
Shipping Temperature: Wet Ice
Origin Pub PMID: PMID: 18241923
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2a
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: The antibody binds the triple helical C1 epitope (GARGLT) of collagen type II.
Application Notes: This anti-collagen type II antibody CIIC1 is an arthritogenic antibody, which induces arthritis in mice (Holmdahl et al., 1986; PMID: 2421741 and Nandakumar et al., 2003; PMID: 12884302). The antibody specificity towards collagen type II was assayed in an ELISA. The antibody was selected for in vivo injection experiments in naive DBA/1 male mice, at no time was any arthritis detected macroscopically after treatment with 0.5, 2 or 5 mg of antibody per animal, however development of synovitis was noticed (Holmdahl et al., 1986; PMID: 2421741). The structure of the Fab fragment was determined by X-ray crystallography. The specificity of the full length CIIC1 antibody and the FV fragment was confirmed by ELISA analysis. Further investigation by using both the full length antibody and a single chain Fv fragment in ELISA experiments revealed that the antibody bound also to other IgG1, IgG2a and IgG2b anti-collagen antibodies (Uysal et al., 2008; PMID: 18241923). The antibody detected collagene type II by western blot analysis (Burkhardt et al., 2005; PMID: 15832289). In vitro studies demonstrated that the antibody together with M2139 antibody suppressed the self-assembly of CII into fibrils (Croxford et al., 2010; PMID: 20662051). This antibody was used for immunohistochemistry on cartilaginous tissue (Vickers et al., 2010; PMID: 20225321).

Product Class:	Purified
Clone ID:	CIIC1
Heavy Chain Modification:	Fc Silent™
Synonyms:	CII; Alpha-1 type II collagen; Collagen alpha-1(II) chain
Amount:	200 μg
Application Codes Clone:	ELISA; Crystallization; in vivo; WB; in vitro; IHC
Shipping Temperature:	Wet Ice
Origin Pub PMID:	PMID: 18241923
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2a
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	The antibody binds the triple helical C1 epitope (GARGLT) of collagen type II.
Application Notes:	This anti-collagen type II antibody CIIC1 is an arthritogenic antibody, which induces arthritis in mice (Holmdahl et al., 1986; PMID: 2421741 and Nandakumar et al., 2003; PMID: 12884302). The antibody specificity towards collagen type II was assayed in an ELISA. The antibody was selected for in vivo injection experiments in naive DBA/1 male mice, at no time was any arthritis detected macroscopically after treatment with 0.5, 2 or 5 mg of antibody per animal, however development of synovitis was noticed (Holmdahl et al., 1986; PMID: 2421741). The structure of the Fab fragment was determined by X-ray crystallography. The specificity of the full length CIIC1 antibody and the FV fragment was confirmed by ELISA analysis. Further investigation by using both the full length antibody and a single chain Fv fragment in ELISA experiments revealed that the antibody bound also to other IgG1, IgG2a and IgG2b anti-collagen antibodies (Uysal et al., 2008; PMID: 18241923). The antibody detected collagene type II by western blot analysis (Burkhardt et al., 2005; PMID: 15832289). In vitro studies demonstrated that the antibody together with M2139 antibody suppressed the self-assembly of CII into fibrils (Croxford et al., 2010; PMID: 20662051). This antibody was used for immunohistochemistry on cartilaginous tissue (Vickers et al., 2010; PMID: 20225321).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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