Mfg Part Number
Ab03293-23.0
Vector Labs Ab03293-23.0 Anti-Galectin-2 [FF06], Rabbit IgG, lambda (200 μg)
Quick Overview
The original antibody was isolated from the antibody library after two rounds of panning against human galectin-2.
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| Product Class: | Purified |
|---|---|
| Clone ID: | FF06 |
| Synonyms: | Gal-2; Beta-galactoside-binding lectin L-14-II; HL14; Lactose-binding lectin 2; S-Lac lectin 2 |
| Amount: | 200 μg |
| Application Codes Clone: | IF; ELISA ; in vivo |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | The antibody binds to human galectin-2. Galectin-2 is a monocyte-expressed carbohydrate-binding lectin, which plays a role in cancer, myocardial infarction, immune response, and gastrointestinal tract diseases. |
| Application Notes: | The scFv fragment was able to bind the recombinant human galectin-2 protein in ELISA experiments. Immunofluorescence analysis on the colon biopsy samples from a healthy donor was performed. The scFv fragment showed significant staining surrounding the crypts of the intestinal tissue. Immunofluorescence analysis on the human colorectal adenocarcinoma cell line LS174T was performed in order to verify the ability of the scFv fragment to recognize natively expressed galectin-2 antigen. The ScFv fragment showed strong cytoplasmic staining. Immunofluorescence analysis on human biopsy samples of Ulcerative Colitis was performed. A stronger intensity signal in the colonic crypts region can be detected in the sections stained with the scFv fragment when compared to the signal observed with an irrelevant antibody. The autoradiography on the intestinal tract of a mouse having colitis has been performed in order to verify the ability of the scFv fragment to recognize the galectin-2 antigen in vivo. A stronger intensity signal could be detected in the colon of the mouse injected with the scFv fragment when compared to the signal in a control mouse injected with an irrelevant antibody (US20190309060). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |