Mfg Part Number
Ab03297-1.159-BS
Vector Labs Ab03297-1.159-BS Anti-Ricin [F8(JOB10)], Mouse IgG1-Fc Fusion, (1 mg)
Quick Overview
The original antibody was generated by immunizing alpacas, followed by library construction and panning on RTA.
$5,162.30
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| Product Class: | Purified |
|---|---|
| Clone ID: | F8(JOB10) |
| Synonyms: | rRNA N-glycosidase; Ricin A chain (EC:3.2.2.22); Ricin B chain |
| Amount: | 1 mg |
| Application Codes Clone: | Crystallography; ELISA; Neutralizing |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 5E1H |
| Buffer Composition: | PBS only. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. Note, this antibody is provided without added preservatives, it is therefore recommed this antibody be handled under sterile conditions. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | The antibody is specific for RTA. The antibody's principal contacts with RTA were centered on β–strand h, with lesser interactions occurring with α–helix B and α–helix D. Ricin is a member of the type II ribosome-inactivating protein (RIP) family of plant toxins. It is a 65 kDa glycoprotein consisting of two subunits, RTA and RTB, joined by a single disulfide bond. |
| Application Notes: | The crystal structure of the VHH antibody in complex with the ricin toxin's enzymatic subunit (RTA) was solved. The specificity of the original format of the antibody was confirmed by ELISA analysis, and the antibody's EC50 for ricin holotoxin was 0.5-0.8 nM. The binding affinity of the VHH fragments to ricin was measured by surface plasmon resonance (Kd= 0.2 nM). In the Vero cell cytotoxicity assay, the VHH had an IC50 of ∼300 nM, thereby classifying it as a weak neutralizing antibody (Rudolph et al., 2016; PMID: 27159829). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |