Mfg Part Number
Ab03297-23.159
Vector Labs Ab03297-23.159 Anti-Ricin [F8(JOB10)], Rabbit IgG-Fc Fusion, (200 μg)
Quick Overview
The original antibody was generated by immunizing alpacas, followed by library construction and panning on RTA.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | F8(JOB10) |
| Synonyms: | rRNA N-glycosidase; Ricin A chain (EC:3.2.2.22); Ricin B chain |
| Amount: | 200 μg |
| Application Codes Clone: | Crystallography; ELISA; Neutralizing |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 5E1H |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | The antibody is specific for RTA. The antibody's principal contacts with RTA were centered on β–strand h, with lesser interactions occurring with α–helix B and α–helix D. Ricin is a member of the type II ribosome-inactivating protein (RIP) family of plant toxins. It is a 65 kDa glycoprotein consisting of two subunits, RTA and RTB, joined by a single disulfide bond. |
| Application Notes: | The crystal structure of the VHH antibody in complex with the ricin toxin's enzymatic subunit (RTA) was solved. The specificity of the original format of the antibody was confirmed by ELISA analysis, and the antibody's EC50 for ricin holotoxin was 0.5-0.8 nM. The binding affinity of the VHH fragments to ricin was measured by surface plasmon resonance (Kd= 0.2 nM). In the Vero cell cytotoxicity assay, the VHH had an IC50 of ∼300 nM, thereby classifying it as a weak neutralizing antibody (Rudolph et al., 2016; PMID: 27159829). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |