Mfg Part Number
Ab03302-10.3
Vector Labs Ab03302-10.3 Anti-GFAP [10G1G11H7], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing mice with purified recombinant GFAP protein.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 10G1G11H7 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | Glial fibrillary acidic protein; CGMCCNO.17407; 6F2 |
| Amount: | 200 μg |
| Application Codes Clone: | WB; ELISA; IHC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | The original antibody recognizes the recombinant protein GFAP. GFAP is a class-III intermediate filament. It is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. |
| Application Notes: | The antibody specifically detected GFAP protein by Western blot analysis. This antibody and the commercial antibody 6F2 were used for immunohistochemistry on astrocytoma, glioma, pituitary and brain tissue and the results were compared. The antibody 10G1G11H7 had accurate staining location on the cell membrane, the staining was clear without non-specific staining, and the background was clean. Further, the results were consistent with the ones of the commercially available antibody GFAP (6F2), indicating that the specificity of the antibodies is equivalent (CN110903389A). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |