Vector Labs Ab03322-1.1 Anti-DOTA (metal-bound) [2D12.5], Mouse IgG1, lambda (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 2D12.5
Synonyms: Tetraxetan; dodecane tetra acetic acid; CHEBI:61028; Yttrium(III) labeled DOTA; Y-DOTA; Yttrium-1 4 7 10-tetraazacyclododecanetetraacetic acid; metal-DOTA
Amount: 200 μg
Application Codes Clone: Radioimmunotherapy
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody binds metal bound DOTA (Tetraxetan) and also shows binding affinity for Yttrium(III) labeled DOTA, (S) nitro benzyl-DOTA chelates and Janus-DOTA (a bivalent form of DOTA). DOTA is a chelating agent which can be labelled with radioactive yttrium ion and even conjugated with monoclonal antibodies for radioimmunotherapy.
Application Notes: The original mouse IgG1 antibody binds DOTA chelates of all the rare earths with approximately Kd ≈ 10(-8) M, making it useful for the capture of probe molecules with a variety of properties (PMID: 15546206). The original IgG1 version of this antibody was used in the pretargeting for radioimmunotherapy in BALB/c mice with KHJJ tumors (PMID: 7954426). In vivo clearance studies have demonstrated that 74-96% of 2D12.5 bound to metal chelates is present 24 hours after administration of the bound complex (US7528235). An engineered version of this antibody with a single cysteine residue at position 54, 55 or 56 of the heavy chain, a site proximal to the protein's binding site, enables formation of weakly electrophilic metal complexes of (S)-2-(4-acrylamidobenzyl)-DOTA (AABD) that may bind and form permanent linkages (PMID: 15546206; 15546207). The heavy chain G54C mutant had a small set of electrophilic ligands, differences in reactivity indicated that the substituents near the electrophilic atom can be important determinants of permanent binding (PMID: 15546207).

Product Class:	Purified
Clone ID:	2D12.5
Synonyms:	Tetraxetan; dodecane tetra acetic acid; CHEBI:61028; Yttrium(III) labeled DOTA; Y-DOTA; Yttrium-1 4 7 10-tetraazacyclododecanetetraacetic acid; metal-DOTA
Amount:	200 μg
Application Codes Clone:	Radioimmunotherapy
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody binds metal bound DOTA (Tetraxetan) and also shows binding affinity for Yttrium(III) labeled DOTA, (S) nitro benzyl-DOTA chelates and Janus-DOTA (a bivalent form of DOTA). DOTA is a chelating agent which can be labelled with radioactive yttrium ion and even conjugated with monoclonal antibodies for radioimmunotherapy.
Application Notes:	The original mouse IgG1 antibody binds DOTA chelates of all the rare earths with approximately Kd ≈ 10(-8) M, making it useful for the capture of probe molecules with a variety of properties (PMID: 15546206). The original IgG1 version of this antibody was used in the pretargeting for radioimmunotherapy in BALB/c mice with KHJJ tumors (PMID: 7954426). In vivo clearance studies have demonstrated that 74-96% of 2D12.5 bound to metal chelates is present 24 hours after administration of the bound complex (US7528235). An engineered version of this antibody with a single cysteine residue at position 54, 55 or 56 of the heavy chain, a site proximal to the protein's binding site, enables formation of weakly electrophilic metal complexes of (S)-2-(4-acrylamidobenzyl)-DOTA (AABD) that may bind and form permanent linkages (PMID: 15546206; 15546207). The heavy chain G54C mutant had a small set of electrophilic ligands, differences in reactivity indicated that the substituents near the electrophilic atom can be important determinants of permanent binding (PMID: 15546207).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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