Mfg Part Number
Ab03329-10.0
Vector Labs Ab03329-10.0 Anti-Bromodeoxyuridine [IIB5], Human IgG1, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing a BALB/c mouse intraperitoneally with BrdU conjugated to Bovine Serum albumin.
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| Product Class: | Purified |
|---|---|
| Clone ID: | IIB5 |
| Synonyms: | BrdU |
| Amount: | 100 μg |
| Application Codes Clone: | FC; IHC; ICC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | The original antibody reacts with bromodeoxyuridine (BrdU) also when incorporated into nuclear DNA. Further, the antibody is known to cross-react with Iododeoxyuridine (IdU). |
| Application Notes: | The binding characteristics of the antibody was determined in a radioimmunoassay using 3H-BrdUrd as a ligand, and the affinity constant was found to be 3.1 x 10^7 L/mol (Schutte et al., 1987; PMID: 3113895). This antibody was used for immunohistochemistry for the detection of bromodeoxyuridine (van Engeland et al., 1997; PMID: 9299167). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |