Mfg Part Number
Ab03335-10.0
Vector Labs Ab03335-10.0 Anti-Cytokeratin 5 and 8 [RCK102], Human IgG1, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing a mouse with cytokeratins from a Human lung cancer cell line (MR21).
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| Product Class: | Purified |
|---|---|
| Clone ID: | RCK102 |
| Synonyms: | K5; CK-8; Keratin K5 + K8; Keratin, type II cytoskeletal 8; Cytokeratin-8; Keratin-8; K8; Type-II keratin Kb8; Keratin, type II cytoskeletal 5; 58 kDa cytokeratin; Cytokeratin-5; CK-5; Keratin-5; Type-II keratin Kb5 |
| Amount: | 100 μg |
| Application Codes Clone: | FC; IHC; WB |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | The original antibody binds to Cytokeratin 5 and 8. |
| Application Notes: | The antibody recognized keratins 5 and 8, and as a result stained virtually all epithelial tissues. This antibody was used for immunohistochemistry on classic SCLC as well as the adenocarcinoma cell lines (Broers et al., 1986; PMID: 2433295). Further, endocervical columnar cells, cervical immature squamous metaplasia, cervical CIN I, CIN II and CIN III reacted with the antibody in immunohistochemistry (Smedts et al., 1990; PMID: 1690513). The antibody detected keratins 5 and 8 by western blot analysis on T 24, HeLa and KB cells (Ramaekers et al., 1987; PMID: 2436934). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |