Mfg Part Number
Ab03364-10.3
Vector Labs Ab03364-10.3 Anti-PD-1 [1G4], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing 6-8 weeks old female BALB/c mice with his-tagged human PD-1 protein.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 1G4 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | CD279; PD1; PD 1; hPD-1; PDCD1; Programmed cell death protein 1; Programmed cell death 1; Protein PD-1; 41-1G4; 1G4-mHvKv |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; FC; therapeutic |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG4 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG4 format for improved compatibility with existing reagents assays and techniques. |
| Specificity Statement: | This antibody binds human PD-1 protein. PD-1 acts as an inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. It delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2. |
| Application Notes: | The initial binding characterization of the antibody was done using ELISA. The binding reactivity of this antibody to human, mouse and rhesus monkey PD-1 expressed on CHO cells was determined using flow cytometry. This antibody was shown to cross react with human and rhesus monkey PD-1 and not mouse PD-1. The binding affinity of the original mouse IgG4 antibody for human PD-1 was determined using surface plasmon resonance and was found to be Kd= 1.07 nM. The anti-tumor effect of this antibody was tested in vivo in a mouse colon cancer model. Murine MC-38 cells (colon adenocarcinoma cells) were injected subcutaneously in B-hPD-1 mice and treated with this antibody. It was reported that this antibody showed good tumor inhibitory effect (CN111763257). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |